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Comprehensive typing and genetic analysis of L. monocytogenes isolates: implication for food safety and antibiotic resistance surveillance

2023-12Conference object Restricted access
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dc.contributor.authorSilva, Adriana
dc.contributor.authorSilva, Vanessa
dc.contributor.authorBorges, Vítor
dc.contributor.authorCoelho, Anabela
dc.contributor.authorBatista, Rita
dc.contributor.authorEsteves, Alexandra
dc.contributor.authorIgrejas, Gilberto
dc.contributor.authorSaraiva, Cristina
dc.contributor.authorGomes, João Paulo
dc.contributor.authorPoeta, Patrícia
dc.date.accessioned2024-02-15T14:48:29Z
dc.date.available2024-02-15T14:48:29Z
dc.date.issued2023-12
dc.descriptionEste trabalho foi realizado em colaboração com o Departamento de Doenças Infecciosas, a University of Trás-os-Montes and Alto Douro, o LAQV-REQUIMTE e o Centro de Ciência Animal e Veterinária (CECAV)pt_PT
dc.description.abstractListeria species are commonly found in various environments and contaminated food, with livestock serving as a significant source of foodborne pathogens. Among these species, Listeria monocytogenes (L. monocytogenes) is particularly noteworthy as it can affect both livestock and humans. Antibiotics are frequently used in food animals for disease treatment and prevention on a large scale. This practice can lead to the selection of antibiotic-resistant bacterial strains, which can then spread to humans through the food chain. Consequently, L. monocytogenes, a ubiquitous foodborne pathogen, has been associated with global outbreaks of foodborne illnesses. To address this concern, the aim of the study was to conduct comprehensive typing and genetic analysis of 13 L. monocytogenes isolates obtained from food and food-processing environments.Among the 13 L. monocytogenes isolates, eight sequence types (ST) were identified: two isolates were identified as belonging to ST9; one as ST155; four as ST3, two as ST121, one as ST8; one as ST87; one as ST1; and one new ST belonging to CC121. Core-genome clustering analysis of L. monocytogenes was made to assess the genetic relatedness among the isolates. The core genome Multilocus Sequence Typing (cgMLST) analysis revealed three genetic clusters of high closely related isolates (≤7 allelic differences (ADs)): cluster 1. Regarding L. monocytogenes typing, ST3 was the most prevalent among the isolates, found in 4 isolates, followed by ST9 and ST121. Some of these isolates, like ST1, ST9 and ST87, were previously associated with human clinical cases. We used Whole Genome Sequencing (WGS) alongside epidemiological data to link strains to human illnesses and potential food sources. Through cgMLST analysis, we identified genetic clusters of closely related isolates, all linked to the same producers. This approach helped us pinpoint common sources of contamination and gain insights into the transmission dynamics of L. monocytogenes in the context of food safety and public health. The escalating antibiotic resistance in Listeria species, particularly in L. monocytogenes, emphasizes the need for heightened surveillance and improved hygiene practices in the food industry to curb the spread of antibiotic resistance and ensure food safety.pt_PT
dc.description.sponsorshipProjeto UIDP/CVT/00772/2020 and LA/P/0059/2020 (FCT). Laboratório Associado para a Química Verde—LAQV o qual é financiado por fundos nacionais da FCT/MCTES (UIDB/50006/2020 and UIDP/50006/2020). Adriana Silva foi financiada pela FCT (Fundação para a Ciência e a Tecnologia) durante o seu doutortamento (Bolsa SFRH/BD/04576/2020).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/9114
dc.language.isoengpt_PT
dc.relationVeterinary and Animal Science Research Centre
dc.relationAssociate Laboratory for Animal and Veterinary Sciences
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
dc.relationSustainable utilization of winery by-products as food supplements in animal production: Fight antibiotic resistance from a food safety
dc.subjectL. monocytogenespt_PT
dc.subjectFood-productspt_PT
dc.subjectFood Safetypt_PT
dc.subjectEpidemiologypt_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleComprehensive typing and genetic analysis of L. monocytogenes isolates: implication for food safety and antibiotic resistance surveillancept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleVeterinary and Animal Science Research Centre
oaire.awardTitleAssociate Laboratory for Animal and Veterinary Sciences
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleAssociated Laboratory for Green Chemistry - Clean Technologies and Processes
oaire.awardTitleSustainable utilization of winery by-products as food supplements in animal production: Fight antibiotic resistance from a food safety
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00772%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/LA%2FP%2F0059%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F50006%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/2020.04576.BD/PT
oaire.citation.conferencePlaceOnlinept_PT
oaire.citation.title3rd international electronic conference on antibiotics (ECA2023) 'Rise of Antibiotic Resistance: Mechanisms Involved and Solutions to Tackle it', 1-15 December 2023pt_PT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamOE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isProjectOfPublication.latestForDiscoveryeccce5f3-585c-46ef-9277-7db5962d45f7

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