Publicação
Unraveling the genome-wide repertoire of the novel chromosomally encoded mcr-8.6 gene variant in Klebsiella michiganensis isolated from manure
| datacite.subject.fos | Ciências Naturais::Ciências Biológicas | |
| dc.contributor.author | Rivière, Rani | |
| dc.contributor.author | Teixeira, Pedro | |
| dc.contributor.author | Silva, Catarina | |
| dc.contributor.author | Ramos, Miguel | |
| dc.contributor.author | Dias, Elsa | |
| dc.contributor.author | Manageiro, Vera | |
| dc.contributor.author | Caniça, Manuela | |
| dc.date.accessioned | 2026-02-09T16:03:15Z | |
| dc.date.available | 2026-02-09T16:03:15Z | |
| dc.date.issued | 2025-12-03 | |
| dc.description.abstract | The increasing rates of colistin resistance worldwide poses a significant threat to public health. While the most commonly described variant is , other variants such as have been detected, typically associated with . However, little is known about the prevalence of in other bacterial species and environmental reservoirs. This study aimed to characterize a novel subvariant identified in a strain isolated from manure in Portugal, collected during an annual longitudinal survey at an Open Air laboratory, as well as to depict its genomic context and potential mobility mechanisms. The strain was subjected to phenotypic susceptibility testing, whole-genome sequencing and hybrid genome assembly. analysis included identification of resistance genes and mobile genetic element. The new gene variant and its genetic environment were characterized. The F731 strain presented susceptibility to colistin with a MIC = 0.25 mg/L, despite carrying a novel subvariant, , which was located within a 61.6 kb chromosomal genomic island. This variant presented 23-24 amino acid substitutions compared to previous characterized MCR-8 proteins. The genomic island also harbored multiple insertion sequences (IS, IS, IS), virulence factors, and metabolic and regulatory proteins, among others. Synteny analysis revealed high sequence identity between this genomic island and both chromosomal and plasmid regions from other bacterial strains isolated from different reservoirs worldwide, indicating prior mobility. Furthermore, other antimicrobial resistance genes were detected [e.g., ', ], but no plasmid replicons were identified. This is the first report of a gene in a , as well as the first occurrence in Portugal. Although F731 remains colistin-susceptible, the presence of a novel chromosomally encoded but located in a mobile genomic island underscores the risk of future horizontal gene transfer. These findings highlight the importance of further monitoring and continued surveillance in environmental and animal compartments in order to track the dissemination of antimicrobial resistance. | eng |
| dc.description.sponsorship | RR, PT, and MR were granted by Agendas/Alianças mobilizadoras para a Reindustrialização (no. 5, SMARTgNOSTICS project). This work was supported by funding from the European Union’s Horizon 2020 Research and Innovation programme under grant agreement no 773830: One Health European Joint Programme (FED-AMR project), and from Agendas/Alianças mobilizadoras para a Reindustrialização (no. 5, SMARTgNOSTICS project) | |
| dc.identifier.citation | Front Microbiol. 2025 Dec 3:16:1673320. doi: 10.3389/fmicb.2025.1673320. eCollection 2025 | |
| dc.identifier.doi | 10.3389/fmicb.2025.1673320 | |
| dc.identifier.eissn | 1664-302X | |
| dc.identifier.pmid | 41415816 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/10872 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Frontiers Media | |
| dc.relation | Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards. | |
| dc.relation.hasversion | https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1673320/full | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.subject | Klebsiella michiganensis | |
| dc.subject | Chromosome-encoded Resistance | |
| dc.subject | Colistin Resistance | |
| dc.subject | Genomic Island | |
| dc.subject | Manure | |
| dc.subject | mcr-8.6 | |
| dc.subject | Mobile Genetic Elements | |
| dc.subject | Resistência aos Antimicrobianos | |
| dc.subject | Portugal | |
| dc.title | Unraveling the genome-wide repertoire of the novel chromosomally encoded mcr-8.6 gene variant in Klebsiella michiganensis isolated from manure | eng |
| dc.type | journal article | |
| dcterms.references | https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2025.1673320/full#supplementary-material | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Promoting One Health in Europe through joint actions on foodborne zoonoses, antimicrobial resistance and emerging microbiological hazards. | |
| oaire.awardURI | info:eu-repo/grantAgreement/EC/H2020/773830/EU | |
| oaire.citation.startPage | 1673320 | |
| oaire.citation.title | Frontiers in Microbiology | |
| oaire.citation.volume | 16 | |
| oaire.fundingStream | H2020 | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| project.funder.identifier | http://doi.org/10.13039/501100008530 | |
| project.funder.name | European Commission | |
| relation.isProjectOfPublication | 94d118fb-33ce-49fa-b1ed-d5bddf63581d | |
| relation.isProjectOfPublication.latestForDiscovery | 94d118fb-33ce-49fa-b1ed-d5bddf63581d |