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Diversity of bacterial communities and genes encoding AMR in different environmental compartments along the food/feed chain

2021-06-09Conference object Restricted access
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dc.contributor.authorManageiro, Vera
dc.contributor.authorCano, Manuela
dc.contributor.authorDias, Elsa
dc.contributor.authorRosado, Tânia
dc.contributor.authorVieira, Patrícia
dc.contributor.authorReis, Lígia
dc.contributor.authorMatias, Rui
dc.contributor.authorRodrigues, João
dc.contributor.authorCoelho, Rodrigo
dc.contributor.authorMenezes, Carina
dc.contributor.authorFerreira, Eugénia
dc.contributor.authorSequeira, António
dc.contributor.authorMoreira, Olga
dc.contributor.authorCaniça, Manuela
dc.date.accessioned2022-07-09T16:33:45Z
dc.date.available2022-07-09T16:33:45Z
dc.date.issued2021-06-09
dc.description.abstractAim: The aim of this study was to determine the microbial biodiversity and naturally occurring antibiotic resistance (AR)-encoding genes background load along the food/feed chain in an open-air agricultural testing catchment (HOAL). Methods: Samples were collected in two time points during the year 2020 from various – but interconnected – environmental compartments of the food/feed chain within an HOAL catchment, such as air, pig feces, manure, soil, water, crops, and feed. All samples were homogenized, diluted and plated in selective media. Colonies were selected andidentification made by MALDI-TOF. Antibiotic susceptibility (AST) was assessed by disc diffusion and/or MIC methods. DNA was extracted for further molecular and genomic characterization. Results: A total of 753 Gram-negative strains were isolated. The highest number of bacteria were from families of Enterobacteriaceae(44.1%), Morganellaceae(15.5%), Moraxellaceae(15.5%), and Pseudomonadaceae(9.3%). Enterobacteriaceae and Pseudomonadaceae were present in all but one of the tested compartments (ground water and feed, respectively). Of notice, 43.1% of Enterobacteriaceae identified were isolated from a selective medium supplemented with colistin 0.5mg/L and 8.7% were selected with cefotaxime 2mg/L. Extended-spectrum -lactamase (ESBL)-producing strains were identified. Regarding Gram-positive bacteria, we identified mainly Enterococcaceae (46.7%), Bacillaceae (30.6%), and Streptococcaceae (13.9%). Pig faces and manure were the compartments with higher bacterial biodiversity. Phenotypic AST revealed the presence of resistances against several AR classes. Based on this data, strains were classified as multidrug-resistant. Conclusions: The investigation of clinically important AR-encoding genes by PCR-amplification and whole-genome-sequencing of selected strains will contribute to clarify the resistome and microbial biodiversity in the tested environmental compartments.pt_PT
dc.description.sponsorshipThis project is funded by One Health EJPpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8148
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectAMRpt_PT
dc.subjectEnvironmental Reservoirspt_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.subjectFood/feed Chainpt_PT
dc.subjectOpen-air Agricultural Testing Catchmentpt_PT
dc.titleDiversity of bacterial communities and genes encoding AMR in different environmental compartments along the food/feed chainpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceCopenhagen, Denmark/ Onlinept_PT
oaire.citation.title3rd One Health European Joint Programme Annual Scientific Meeting, 9-11 June 2021pt_PT
rcaap.rightsclosedAccesspt_PT
rcaap.typeconferenceObjectpt_PT

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