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  • Diversity of bacterial communities and genes encoding AMR in different environmental compartments along the food/feed chain
    Publication . Manageiro, Vera; Cano, Manuela; Dias, Elsa; Rosado, Tânia; Vieira, Patrícia; Reis, Lígia; Matias, Rui; Rodrigues, João; Coelho, Rodrigo; Menezes, Carina; Ferreira, Eugénia; Sequeira, António; Moreira, Olga; Caniça, Manuela
    Aim: The aim of this study was to determine the microbial biodiversity and naturally occurring antibiotic resistance (AR)-encoding genes background load along the food/feed chain in an open-air agricultural testing catchment (HOAL). Methods: Samples were collected in two time points during the year 2020 from various – but interconnected – environmental compartments of the food/feed chain within an HOAL catchment, such as air, pig feces, manure, soil, water, crops, and feed. All samples were homogenized, diluted and plated in selective media. Colonies were selected andidentification made by MALDI-TOF. Antibiotic susceptibility (AST) was assessed by disc diffusion and/or MIC methods. DNA was extracted for further molecular and genomic characterization. Results: A total of 753 Gram-negative strains were isolated. The highest number of bacteria were from families of Enterobacteriaceae(44.1%), Morganellaceae(15.5%), Moraxellaceae(15.5%), and Pseudomonadaceae(9.3%). Enterobacteriaceae and Pseudomonadaceae were present in all but one of the tested compartments (ground water and feed, respectively). Of notice, 43.1% of Enterobacteriaceae identified were isolated from a selective medium supplemented with colistin 0.5mg/L and 8.7% were selected with cefotaxime 2mg/L. Extended-spectrum -lactamase (ESBL)-producing strains were identified. Regarding Gram-positive bacteria, we identified mainly Enterococcaceae (46.7%), Bacillaceae (30.6%), and Streptococcaceae (13.9%). Pig faces and manure were the compartments with higher bacterial biodiversity. Phenotypic AST revealed the presence of resistances against several AR classes. Based on this data, strains were classified as multidrug-resistant. Conclusions: The investigation of clinically important AR-encoding genes by PCR-amplification and whole-genome-sequencing of selected strains will contribute to clarify the resistome and microbial biodiversity in the tested environmental compartments.
    2021-06-09Conference object Restricted access Show more
  • First Report of Echinococcus ortleppi in Free-Living Wild Boar (Sus scrofa) from Portugal
    Publication . Mateus, Teresa Letra; Gargaté, Maria João; Vilares, Anabela; Ferreira, Idalina; Rodrigues, Manuela; Coelho, Catarina; Vieira-Pinto, Madalena
    Cystic echinococcosis (CE) is a zoonosis that is prevalent worldwide. It is considered endemic in Portugal but few studies have been performed on Echinococcus granulosus sensu lato and their hosts. In this study, CE cysts are reported for the first time in a free-living wild boar (Sus scrofa) in Portugal. The presence of the metacestodes in the liver of the wild boar was identified by morphological features, microscopic examination and molecular analysis. The sequencing of part of the DNA nuclear ribosomal internal transcribed spacer-1 (ITS-1) region revealed a G5 genotype that presently corresponds to Echinococcus ortleppi. This is the first report of E. ortleppi in Portugal and to the best of the authors’ knowledge, in Europe. These results suggest that wild boar may be a host of CE, namely, crossing the livestock–wildlife interface, which has important public health implications. Wildlife reservoirs must be taken into account as CE hosts and surveillance of game as well as health education for hunters should be implemented using a One Health approach, with implementation of feasible and tailor-made control strategies, namely, proper elimination of byproducts in the field.
    2021-06-09Journal article Open access Show more