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Occurrence, genetic diversity and antibiotic resistance of Arcobacter sp. in a dairy plant

2017-10Journal article Restricted access
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dc.contributor.authorFerreira, S
dc.contributor.authorOleastro, M
dc.contributor.authorDomingues, F C
dc.date.accessioned2018-02-20T16:04:18Z
dc.date.available2018-02-20T16:04:18Z
dc.date.issued2017-10
dc.description.abstractAims: The aim of this study was to evaluate the occurrence, diversity and resistance to antibiotics of Arcobacter sp. in a dairy plant samples. Methods and Results: A total of 75 samples from dairy plant surfaces and materials and several food products collected in different steps of the cheese production process were analysed by culture, under aerobic and microaerobic atmospheric conditions, and by enrichment molecular detection. Isolates were identified and genotyped by ERIC-PCR, and their susceptibility to nine antibiotics was evaluated by agar dilution. Global prevalence of Arcobacter sp. was 42 7%, where 20 of the 42 food samples analysed were positive for A. butzleri by both culture and molecular detection, one for A. marinus by culture and one for A. cryaerophilus by molecular detection only; 10 of the 30 analysed materials and plant surfaces were positive for A. butzleri. All A. butzleri isolates were resistant to nalidixic acid and showed high resistance rates to ampicillin (56 2%) and cefotaxime (97 9%), being all strains susceptible to gentamicin and erythromycin. Conclusions: Contamination of dairy plant environment with A. butzleri and its progression along cheese production process were observed, however, the cheese ripening process may have a relevant role in the reduction of the contamination. Significance and Impact of the Study: This study showed the presence of Arcobacter sp. in a dairy plant, displaying its high prevalence and genetic diversity and highlighting its high resistance rates. The data obtained could contribute to further acknowledge the Arcobacter food contamination as a potential health hazard.pt_PT
dc.description.sponsorshipSusana Ferreira was supported by a fellowship (SFRH/BPD/101959/2014) from FCT—Foundation for Science and Technology and co-financed by European Social Fund. This work was supported by FEDER funds through the POCI—COMPETE 2020—Operational Programme Competitiveness and Internationalisation in Axis I — Strengthening research, technological development and innovation (Project POCI-01-0145-FEDER-007491) and National Funds by FCT—Foundation for Science and Technology (Project UID/Multi /00709/2013). Capillary sequencing was performed at Unidade de Tecnologia e Inovação (Departamento de Genetica Humana, Instituto Nacional de Saude Dr Ricardo Jorge, Lisbon, Portugal).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Appl Microbiol. 2017 Oct;123(4):1019-1026. doi: 10.1111/jam.13538. Epub 2017 Aug 30.pt_PT
dc.identifier.doi10.1111/jam.13538pt_PT
dc.identifier.issn1364-5072
dc.identifier.urihttp://hdl.handle.net/10400.18/4999
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherWiley/The Society for Applied Microbiologypt_PT
dc.relation.publisherversionhttp://dx.doi.org/10.1111/jam.13538pt_PT
dc.subjectArcobacter sp.pt_PT
dc.subjectDairy Plantpt_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titleOccurrence, genetic diversity and antibiotic resistance of Arcobacter sp. in a dairy plantpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.citation.endPage1026pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage1019pt_PT
oaire.citation.titleJournal of Applied Microbiologypt_PT
oaire.citation.volume123pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationf5e75317-d96b-4bfe-97b5-3912e4125430
relation.isProjectOfPublication.latestForDiscoveryf5e75317-d96b-4bfe-97b5-3912e4125430

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