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Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii

2018-11-10Journal article Restricted access
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dc.contributor.authorMargos, G.
dc.contributor.authorBecker, N.S.
dc.contributor.authorFingerle, V.
dc.contributor.authorSing, A.
dc.contributor.authorRamos, J.A.
dc.contributor.authorLopes de Carvalho, I.
dc.contributor.authorNorte, A.C.
dc.date.accessioned2019-02-22T17:57:23Z
dc.date.available2019-02-22T17:57:23Z
dc.date.issued2018-11-10
dc.description.abstractBorrelia burgdorferi sensu lato comprises a species complex of tick-transmitted bacteria that includes the agents of human Lyme borreliosis. Borrelia turdi is a genospecies of this complex that exists in cryptic transmission cycles mainly between ornithophilic tick vectors and their avian hosts. The species has been originally discovered in avian transmission cycles in Asia but has increasingly been found in Europe. Next generation sequencing was used to sequence the genome of B. turdi isolates obtained from ticks feeding on birds in Portugal to better understand the evolution and phylogenetic relationship of this avian and ornithophilic tick-associated genospecies. Here we use draft genomes of these B. turdi isolates for comparative analysis and to determine the taxonomic position within the B. burgdorferi s.l. species complex. The main chromosomes showed a maximum similarity of 93% to other Borrelia species whilst most plasmids had lower similarities. All three isolates had nine or 10 plasmids and, interestingly, one plasmid with a novel partitioning protein; this plasmid was termed lp30. Phylogenetic analysis of multilocus sequence typing housekeeping genes and 113 single copy orthologous genes revealed that the isolates clustered according to their classification as B. turdi. In phylogenies generated from these 113 genes the isolates cluster together with other Eurasian genospecies and form a sister clade to the avian associated B. garinii and the rodent associated B. bavariensis. These findings show that Borrelia species maintained in cryptic ecological cycles need to be included to fully understand the complex ecology and evolutionary history of this bacterial species complex.pt_PT
dc.description.sponsorshipThis work was supported by the Robert-Koch-Institute through funding of the National Reference Center for Borrelia, by Fundação para a Ciência e a Tecnologia - FCT (MARE – UID/MAR/04292/2013; SFRH/BPD/108197/2015) and by the Portuguese National Institute of Health.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMol Phylogenet Evol. 2019 Feb;131:93-98. doi: 10.1016/j.ympev.2018.10.044. Epub 2018 Nov 10pt_PT
dc.identifier.doi10.1016/j.ympev.2018.10.044pt_PT
dc.identifier.issn1055-7903
dc.identifier.urihttp://hdl.handle.net/10400.18/5933
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/ Academic Presspt_PT
dc.relation.publisherversionhttps://linkinghub.elsevier.com/retrieve/pii/S1055-7903(18)30252-5pt_PT
dc.subjectBirdpt_PT
dc.subjectBorrelia burgdorferi sensu latopt_PT
dc.subjectBorrelia turdipt_PT
dc.subjectHost Associationpt_PT
dc.subjectIlluminapt_PT
dc.subjectSPAdespt_PT
dc.subjectGenome Assemblypt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleCore genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia gariniipt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F04292%2F2013/PT
oaire.citation.endPage98pt_PT
oaire.citation.startPage93pt_PT
oaire.citation.titleMolecular Phylogenetics and Evolutionpt_PT
oaire.citation.volume131pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication0dd4a5cd-2c09-4417-9190-3de42fbc5f7c
relation.isProjectOfPublication.latestForDiscovery0dd4a5cd-2c09-4417-9190-3de42fbc5f7c

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