Shorter p53 isoform expression through na Internal Ribosome Entry Site (IRES) in p53 mRNA

Neves, Ana Rita; Lacerda, Rafaela; Marques-Ramos, Ana; Romão, Luísa; Matsuda, M; Candeias, Marco

http://hdl.handle.net/10400.18/5169

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
Poster for SPGH meeting_Ana Neves.pdf		1.25 MB	Adobe PDF	Download

Send Feedback

Authors

Abstract(s)

The tumour suppressor p53 gene is one of the most studied cancer-related genes. So far, many p53 isoforms have been identified either resulting from alternative splicing, alternative translation or alternative promoter usage. It is known that cap-dependent translation is repressed under stress conditions to preserve energy. Therefore, other translational mechanisms are required to keep the synthesis of stress-response proteins. Internal Ribosome Entry Sites (IRESes) were first discovered in viruses, and then observed in eukaryotes, as secondary structures present in RNA that were capable of recruiting ribosomes to the vicinity of an initiation codon inserted in an optimal environment allowing cap-independent translation of mRNAs. Translation of Δ40p53, a p53 isoform, is one example of this non-canonical mechanism due to the presence of an IRES near an alternative initiation codon (AUG40). Here, we will present a new IRES in p53 mRNA, including details on the localization and regulation of this IRES under normal and stress conditions.