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Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study

2013-10Journal article Restricted access
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dc.contributor.authorReusken, C.
dc.contributor.authorHaagmans, B.
dc.contributor.authorGutierrez, C.
dc.contributor.authorGodeke, G.J.
dc.contributor.authorMeyer, B.
dc.contributor.authorMuth, D.
dc.contributor.authorRaj, V.S.
dc.contributor.authorSmits-De Vries, L.
dc.contributor.authorCorman, V.M.
dc.contributor.authorDrexler, J.F.
dc.contributor.authorSmits, S.L.
dc.contributor.authorSousa, R.
dc.contributor.authorvan Beek, J.
dc.contributor.authorNowotny, N.
dc.contributor.authorvan Maanen, K.
dc.contributor.authorHidalgo-Hermoso, E.
dc.contributor.authorBosch, B.J.
dc.contributor.authorRottier, P.
dc.contributor.authorOsterhaus, A.
dc.contributor.authorGortázar-Schmidt, C.
dc.contributor.authorDrosten, C.
dc.contributor.authorKoopmans, M.
dc.date.accessioned2016-05-25T13:33:32Z
dc.date.available2016-05-25T13:33:32Z
dc.date.issued2013-10
dc.description.abstractA new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. METHODS: We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. FINDINGS: 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. INTERPRETATION: MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection. FUNDING: European Union, European Centre For Disease Prevention and Control, Deutsche Forschungsgemeinschaft.pt_PT
dc.description.sponsorshipContributions to the study were funded through the European Union FP7 projects EMPERIE (contract number 223498; to BLH, SLS, AO, CD) and ANTIGONE (contract number 278976; to CG, CD, MPGK, AO). Work in Bonn was also funded by Deutsche Forschungsgemeinschaft (DFG grant DR772/3-1 to CD).pt_PT
dc.identifier.citationLancet Infect Dis. 2013 Oct;13(10):859-66. doi: 10.1016/S1473-3099(13)70164-6. Epub 2013 Aug 9.pt_PT
dc.identifier.doi10.1016/51473-3099(13)40164-6pt_PT
dc.identifier.issn1473-3099
dc.identifier.urihttp://hdl.handle.net/10400.18/3831
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier: Lancetpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S1473309913701646pt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.subjectMiddle East Respiratory Syndromept_PT
dc.subjectCoronaviruspt_PT
dc.subjectDromedary Camelspt_PT
dc.titleMiddle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage866pt_PT
oaire.citation.startPage859pt_PT
oaire.citation.titleLancet Infectious Diseasespt_PT
oaire.citation.volume13(10)pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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