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Effect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureus

2023-02-23Journal article Open access
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dc.contributor.authorGaálová-Radochová, Barbora
dc.contributor.authorKendra, Samuel
dc.contributor.authorJordao, Luisa
dc.contributor.authorKursawe, Laura
dc.contributor.authorKikhney, Judith
dc.contributor.authorMoter, Annette
dc.contributor.authorBujdáková, Helena
dc.date.accessioned2023-10-06T13:16:08Z
dc.date.available2023-10-06T13:16:08Z
dc.date.issued2023-02-23
dc.descriptionThis article belongs to the Special Issue Microbial Biofilms, Antimicrobials, and Virulence Determinants.pt_PT
dc.description.abstractThe natural bioactive molecule farnesol (FAR) is widely studied mainly for its antibiofilm and antimicrobial properties. In addition, it increases the effectiveness of some antimicrobial substances, which makes it interesting for the development of combined therapy. In the present work, the effect of FAR either alone or in combination with oxacillin (OXA) on mixed biofilms formed by clinically relevant pathogens, Candida albicans and Staphylococcus aureus, was studied. S. aureus isolates used for biofilm formation originated from blood cultures and central venous catheters (CVC) were characterized in terms of antimicrobial resistance. The minimal biofilm inhibitory concentration (MBIC50) for FAR of 48 h mixed biofilms formed by the C. albicans and methicillin-sensitive S. aureus (MSSA) was determined to be 125 M, and for the mixed biofilms with methicillin-resistant S. aureus (MRSA) was determined to be 250 M. Treatment of mixed biofilms with OXA (2 mg/mL) showed 4% inhibition; however, the combination of OXA (2 mg/mL) and FAR (300 M) resulted in 80% inhibition of biofilms. In addition, planktonic cells of S. aureus exhibited an increased susceptibility to OXA, cefoxitin and kanamycin in the presence of FAR (150 and 300 M). Scanning electron microscopy (SEM) micrographs confirmed patchy biofilm and lack of candidal hyphae in the samples treated with FAR and FAR/OXA in comparison to control and mixed biofilms treated only with OXA. Intriguingly, in a pilot experiment using fluorescence in situ hybridization (FISH), considerable differences in activity (as indicated by ribosome content) of staphylococcal cells were detected. While the activity rate of the staphylococci in mixed biofilms treated with FAR was high, no FISH-positive signal for staphylococcal cells was found in the biofilm treated with FAR/OXA.pt_PT
dc.description.sponsorshipThis research was funded by the Slovak Research and Development Agency under contracts of SK-PT-18-0006 as part of the Bilateral Cooperation Program (2019–2022), APVV-21-0302 and APVV-18-0075. This work was also supported by the EU Grant number 952398—CEMBO, Call: H2020-WIDESPREAD-05-2020—Twinning.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationAntibiotics (Basel). 2023 Feb 23;12(3):441. doi: 10.3390/antibiotics12030441pt_PT
dc.identifier.doi10.3390/antibiotics12030441pt_PT
dc.identifier.issn2079-6382
dc.identifier.urihttp://hdl.handle.net/10400.18/8667
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relationExcellence in the research area of Combating and evaluation of mixed biofilms.
dc.relation.publisherversionhttps://www.mdpi.com/2079-6382/12/3/441pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectMethicillin-resistant/sensitive S. aureuspt_PT
dc.subjectBiofilms of C. albicans-MSSA/MRSApt_PT
dc.subjectResistancept_PT
dc.subjectFarnesolpt_PT
dc.subjectEnhancing Effectpt_PT
dc.subjectCell Viabilitypt_PT
dc.subjectMicroscopypt_PT
dc.titleEffect of Quorum Sensing Molecule Farnesol on Mixed Biofilms of Candida albicans and Staphylococcus aureuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleExcellence in the research area of Combating and evaluation of mixed biofilms.
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/952398/EU
oaire.citation.issue3pt_PT
oaire.citation.startPage441pt_PT
oaire.citation.titleAntibioticspt_PT
oaire.citation.volume12pt_PT
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameEuropean Commission
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication0a335fe8-54fc-4397-b4a0-1614f3161008
relation.isProjectOfPublication.latestForDiscovery0a335fe8-54fc-4397-b4a0-1614f3161008

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