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Biocompatibility evaluation of CeO2 nanoparticles to be employed as nanodrugs in brain cancer nanomedicine

2023-02-23Conference object Open access
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dc.contributor.authorFernández-Bertólez, Natália
dc.contributor.authorTouzani, Assia
dc.contributor.authorMartínez, L.
dc.contributor.authorReis, Ana Teresa
dc.contributor.authorFraga, Sónia
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorCosta, Carla
dc.contributor.authorPásaro, Eduardo
dc.contributor.authorLaffon, Blanca
dc.contributor.authorValdiglesias, Vanessa
dc.date.accessioned2024-02-12T10:18:12Z
dc.date.available2024-02-12T10:18:12Z
dc.date.issued2023-02-23
dc.descriptionAbstract publicado em: Book of abstracts. https://www.nano2clinic.eu/sites/default/files/downloads/BoA_2nd_STSM_Conference_DOI.pdfpt_PT
dc.description.abstractCerium dioxide nanoparticles (CeO2NP) have recently gained attention for their unique structure-dependent properties, antioxidant enzyme-like behaviour, ROS scavenging activity and great potential for biomedical applications. In addition to their antioxidant and anti-inflammatory activity, CeO2NP are also known to exhibit anticancer potential, providing an attractive opportunity for use in cancer therapy, as a pharmacological agent and/or in drug/gene delivery systems [1]. Therefore, the main objective of this STSM was to evaluate the cytotoxic and genotoxic effects on human glioblastoma A172 cells exposed for 3, 24 and 48h to CeO2NP (1- 100µg/ml), to verify their safety to be used as possible nanomedicines for brain cancer treatment, specifically glioblastoma [2]. In addition, cell-specific differences in nanoceria effect were evaluated by comparing the results obtained with those observed in human neuronal SH-SY5Y cells exposed under the same experimental conditions. After carrying out the physicochemical characterization and analysing the cellular uptake of the CeO2NP, potential alterations in cell viability (MTT assay) and induction of DNA double-strand breaks (γH2AX assay) caused by the exposure were determined. The possible NP interference with assay methodologies was previously addressed and eliminated when necessary. Results obtained showed that, although there was a significant dose- and time-dependent internalization of NP by both cell types, nanoceria induced scarce cytotoxicity or genotoxicity in both cell lines, being restricted to the highest doses and longer exposure time tested. In general, data obtained suggest a high biocompatibility of CeO2NP under the tested conditions, except for glioblastoma cells exposed for 48h from 25 to 100µg/ml. These results provide a better understanding of the CeO2NP interaction with nervous system cells and their possible adverse effects. However, further studies are necessary to delve into the differential behaviour of these NP depending on the nervous cell type tested.pt_PT
dc.description.sponsorshipFunding: Spanish Ministry of Science and Innovation MCIN/AEI/10.13039/501100011033 (Grant PID2020-114908GA-I00); Xunta de Galicia (ED431B 2022/16); CICA-Disrupting Project 2021SEM‐B2; Ministry of Education, Culture and Sport (BEAGAL18/00142 to V.V.); and FCT (SFRH/BPD/122112/2016 to A.T.R). This communication is based upon work from COST Action “Cancer Nanomedicine - from the Bench to the Bedside” CA17140, supported by COST (European Cooperation in Science and Technology) for the Short-Term Scientific Mission grant (E-COST-GRANT-CA17140-a2a8329b, to N.F-B.) Funding: Spanish Ministry of Science and Innovation MCIN/AEI/10.13039/501100011033 (Grant PID2020-114908GA I00); Xunta de Galicia (ED431B 2022/16); CICA-Disrupting Project 2021SEM‐B2; Ministry of Education, Culture and Sport (BEAGAL18/00142 to V.V.); and FCT (SFRH/BPD/122112/2016 to A.T.R). This communication is based upon work from COST Action “Cancer Nanomedicine - from the Bench to the Bedside” CA17140, supported by COST (European Cooperation in Science and Technology) for the Short-Term Scientific Mission grant (E-COST-GRANT-CA17140-a2a8329b, to N.F-B.)pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/9078
dc.language.isoengpt_PT
dc.relationA novelty study on the human uptake, genotoxicity and immunotoxicity of nanoparticles and legacy contaminants mixtures
dc.subjectNanoparticlespt_PT
dc.subjectCeO2 Nanoparticlespt_PT
dc.subjectBrain Cancerpt_PT
dc.subjectNanomedicinept_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleBiocompatibility evaluation of CeO2 nanoparticles to be employed as nanodrugs in brain cancer nanomedicinept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleA novelty study on the human uptake, genotoxicity and immunotoxicity of nanoparticles and legacy contaminants mixtures
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F122112%2F2016/PT
oaire.citation.title2nd CA17140 STSM Virtual Conference, 23 February 2023pt_PT
person.familyNameLemos Pereira Saúde Reis
person.givenNameAna Teresa
person.identifier.ciencia-id5E14-031E-EAE3
person.identifier.orcid0000-0001-7150-997X
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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relation.isAuthorOfPublication.latestForDiscoveryb735213d-ccf3-4f45-ad24-db085e06f38b
relation.isProjectOfPublicationdbbc9b73-85f0-4bba-b64e-3624dc6b4d90
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