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Toxicity assessment of industrial engineered and airborne process-generated nanoparticles in a 3D human airway epithelial in vitro model

2021-05Journal article Restricted access
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dc.contributor.authorBessa, Maria João
dc.contributor.authorBrandão, Fátima
dc.contributor.authorFokkens, Paul
dc.contributor.authorCassee, Flemming R.
dc.contributor.authorSalmatonidis, Apostolos
dc.contributor.authorViana, Mar
dc.contributor.authorVulpoi, Adriana
dc.contributor.authorSimon, Simion
dc.contributor.authorMonfort, Eliseo
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorFraga, Sónia
dc.date.accessioned2022-01-27T17:01:24Z
dc.date.available2022-01-27T17:01:24Z
dc.date.issued2021-05
dc.description.abstractThe advanced ceramic technology has been pointed out as a potentially relevant case of occupational exposure to nanoparticles (NP). Not only when nanoscale powders are being used for production, but also in the high-temperature processing of ceramic materials there is also a high potential for NP release into the workplace environment. In vitro toxicity of engineered NP (ENP) [antimony tin oxide (Sb2O3•SnO2; ATO); zirconium oxide (ZrO2)], as well as process-generated NP (PGNP), and fine particles (PGFP), was assessed in MucilAir™ cultures at air-liquid interface (ALI). Cultures were exposed during three consecutive days to varying doses of the aerosolized NP. General cytotoxicity [lactate dehydrogenase (LDH) release, WST-1 metabolization], (oxidative) DNA damage, and the levels of pro-inflammatory mediators (IL-8 and MCP-1) were assessed. Data revealed that ENP (5.56 µg ATO/cm2 and 10.98 µg ZrO2/cm2) only caused mild cytotoxicity at early timepoints (24 h), whereas cells seemed to recover quickly since no significant changes in cytotoxicity were observed at late timepoints (72 h). No meaningful effects of the ENP were observed regarding DNA damage and cytokine levels. PGFP affected cell viability at dose levels as low as ∼9 µg/cm2, which was not seen for PGNP. However, exposure to PGNP (∼4.5 µg/cm2) caused an increase in oxidative DNA damage. These results indicated that PGFP and PGNP exhibit higher toxicity potential than ENP in mass per area unit. However, the presence of a mucociliary apparatus, as it occurs in vivo as a defense mechanism, seems to considerably attenuate the observed toxic effects. Our findings highlight the potential hazard associated with exposure to incidental NP in industrial settings.pt_PT
dc.description.sponsorshipThe current work was carried out in the framework of the CERASAFE project (www.cerasafe.eu), with the support of ERA-NET SIINN (project id:16) and the Portuguese Foundation for Science and Technology (FCT; SIINN/0004/ 2014). This work was also supported by the NanoBioBarriers project (PTDC/MED-TOX/31162/2017), co-financed by the Operational Program for Competitiveness and Internationalization (POCI) through European Regional Development Funds (FEDER/FNR) and FCT; Spanish Ministry of Science and Innovation (projects PCIN-2015-173-C02-01 and CEX2018-000794-S-Severo Ochoa) and by the Romanian National Authority for Scientific Research and Innovation (CCCDI-UEFISCDI, project number 29/2016 within PNCDI III). Thanks are also due to FCT/MCTES for the financial support to EPIUnit (UIDB/04750/2020). M.J. Bessa (SFRH/BD/120646/ 2016) and F. Brand~ao (SFRH/BD/101060/2014) are recipients of FCT PhD scholarships under the framework of Human Capital Operating Program (POCH) and European Union funding.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNanotoxicology. 2021 May;15(4):542-557. doi: 10.1080/17435390.2021.1897698. Epub 2021 Mar 18.pt_PT
dc.identifier.doi10.1080/17435390.2021.1897698pt_PT
dc.identifier.issn1743-5390
dc.identifier.urihttp://hdl.handle.net/10400.18/7883
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor and Francispt_PT
dc.relationIDB/04750/2020pt_PT
dc.relationSAFE PRODUCTION AND USE OF NANOMATERIALS IN THE CERAMIC INDUSTRY
dc.relationAssessing the toxicity of airborne nanoceramics in vitro and in vivo- contribution to the safe production and use of nanomaterials in the ceramic industry
dc.relationEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
dc.relation.publisherversionhttps://www.tandfonline.com/doi/abs/10.1080/17435390.2021.1897698?journalCode=inan20pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectCeramic Technologypt_PT
dc.subjectMucilAir™pt_PT
dc.subjectEngineered Nanoparticlespt_PT
dc.subjectProcess-generated Nanoparticlespt_PT
dc.subjectThermal Sprayingpt_PT
dc.subjectNanoparticlespt_PT
dc.subjectAr e Saúde Ocupacionalpt_PT
dc.subjectToxicologiapt_PT
dc.titleToxicity assessment of industrial engineered and airborne process-generated nanoparticles in a 3D human airway epithelial in vitro modelpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSAFE PRODUCTION AND USE OF NANOMATERIALS IN THE CERAMIC INDUSTRY
oaire.awardTitleAssessing the toxicity of airborne nanoceramics in vitro and in vivo- contribution to the safe production and use of nanomaterials in the ceramic industry
oaire.awardTitleEstablishing grouping strategies according to toxicity and biological effects for silica based manufactured nanomaterials
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/SIINN%2F0004%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FMED-TOX%2F31162%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F120646%2F2016/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/OE/SFRH%2FBD%2F101060%2F2014/PT
oaire.citation.endPage557pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage542pt_PT
oaire.citation.titleNanotoxicologypt_PT
oaire.citation.volume15pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamOE
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project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
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