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An Overview of Monkeypox Virus Detection in Different Clinical Samples and Analysis of Temporal Viral Load Dynamics

2024-12Research article Open access
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dc.contributor.authorCordeiro, Rita
dc.contributor.authorPelerito, Ana
dc.contributor.authorde Carvalho, Isabel Lopes
dc.contributor.authorLopo, Sílvia
dc.contributor.authorNeves, Raquel
dc.contributor.authorRocha, Raquel
dc.contributor.authorPalminha, Paula
dc.contributor.authorVerdasca, Nuno
dc.contributor.authorPalhinhas, Cláudia
dc.contributor.authorBorrego, Maria José
dc.contributor.authorManita, Carla
dc.contributor.authorFerreira, Idalina
dc.contributor.authorBettencourt, Célia
dc.contributor.authorVieira, Patrícia
dc.contributor.authorSilva, Sónia
dc.contributor.authorÁgua-Doce, Ivone
dc.contributor.authorRoque, Carla
dc.contributor.authorCordeiro, Dora
dc.contributor.authorBrondani, Greice
dc.contributor.authorSantos, João Almeida
dc.contributor.authorMartins, Susana
dc.contributor.authorRodrigues, Irene
dc.contributor.authorRibeiro, Carlos
dc.contributor.authorNúncio, Maria Sofia
dc.contributor.authorGomes, João Paulo
dc.contributor.authorBatista, Fernando da Conceição
dc.date.accessioned2025-03-17T14:35:56Z
dc.date.available2025-03-17T14:35:56Z
dc.date.issued2024-12
dc.description.abstractMpox is a zoonotic disease caused by the Monkeypox virus (MPXV), and since May 2022, tens of thousands of cases have been reported in non-endemic countries. We aimed to evaluate the suitability of different sample types for mpox diagnostic and assess the temporal dynamics of viral load. We evaluated 1914 samples from 953 laboratory-confirmed cases. The positivity rate was higher for lesion (91.3%) and rectal swabs (86.1%) when compared with oropharyngeal swabs (69.5%) and urines (41.2%), indicating higher viral loads for the former. Supporting this, lesion and rectal swabs showed lower median PCR C values (C = 23 and C = 24), compared to oropharyngeal swabs and urines (C = 31). Stable MPXV loads were observed in swabs from lesions up to 30 days after symptoms onset, contrasting with a considerable decrease in viral load in rectal and oropharyngeal swabs. Overall, these results point to lesion swabs as the most suitable samples for detecting MPXV in the 2022-2023 multicountry outbreak and show comparable accuracy to rectal swabs up to 8 days after symptoms onset. These findings, together with the observation that about 5% of patients were diagnosed through oropharyngeal swabs while having negative lesions, suggest that multisite testing should be performed to increase diagnostic sensitivity.
dc.identifier.citationJ Med Virol. 2024 Dec;96(12):e70104. doi: 10.1002/jmv.70104
dc.identifier.doi10.1002/jmv.70104
dc.identifier.issn0146-6615
dc.identifier.pmid39654358
dc.identifier.urihttp://hdl.handle.net/10400.18/10442
dc.language.isoeng
dc.peerreviewedyes
dc.publisherWiley
dc.relation.hasversionhttps://onlinelibrary.wiley.com/doi/10.1002/jmv.70104
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectCt Values
dc.subjectMonkeypox Virus
dc.subjectClinical Samples
dc.subjectPositive Rate
dc.subjectViral Clearance
dc.subjectViral Load
dc.subjectPortugal
dc.subjectInfecções Sistémicas e Zoonoses
dc.titleAn Overview of Monkeypox Virus Detection in Different Clinical Samples and Analysis of Temporal Viral Load Dynamicspor
dc.typeresearch article
dspace.entity.typePublication
oaire.citation.issue12
oaire.citation.startPagee70104
oaire.citation.titleJournal of Medical Virology
oaire.citation.volume96
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85

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