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UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants

2018-02Journal article Restricted access
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dc.contributor.authorOliveira, Ana Sofia
dc.contributor.authorRibeiro-Santos, Regiane
dc.contributor.authorRamos, Fernando
dc.contributor.authorCastilho, Maria Conceição
dc.contributor.authorSanches-Silva, Ana
dc.date.accessioned2019-03-28T18:48:43Z
dc.date.available2019-03-28T18:48:43Z
dc.date.issued2018-02
dc.description.abstractThe aim of this paper was to develop and validate a method of ultra high-performance liquid chromatography coupled with diode array detector (UHPLC-DAD), which allows quantifying simultaneously 19 phenolic compounds in aromatic plants. Twenty-one fresh and dried aromatic plants were extracted with methanol before their UHPLC-DAD analysis. The method was validated regarding specificity, working range, linearity, limit of detection (LOD) and quantification (LOQ), precision, and accuracy. The developed method allows detecting and quantifying the phenolic compounds at low concentrations (LOD < 0.15 μg/mL, except for carnosol and carnosic acid). The relative standard deviation obtained was lower than 5.73 and 8.75%, for repeatability and intermediate precision, respectively. The proposed method is straightforward and sensitive, with good precision and accuracy. Dried thyme presented the highest diversity of phenolic compounds and rosmarinic acid was present in most of the samples. This analytical tool is a valid alternative to conventional methods to quantify phenolic compounds in aromatic plants or in their extracts.pt_PT
dc.description.sponsorshipThis study was funded by the Foundation for Science and Technology (FCT) (PTDC/AGRTEC/3366/2012), COMPETE Program (FCOMP-01-0124-FEDER-028015), and the Brazilian Federal Agency for Support and Evaluation of Graduate Education (CAPES) (BEX 8754/14-4).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFood Anal. Methods. 2018;11:440-450. doi:10.1007/s12161-017-1015-ypt_PT
dc.identifier.doi10.1007/s12161-017-1015-ypt_PT
dc.identifier.issn1936-9751
dc.identifier.urihttp://hdl.handle.net/10400.18/6339
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringerpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007%2Fs12161-017-1015-ypt_PT
dc.subjectPhenolic Compoundspt_PT
dc.subjectUHPLCpt_PT
dc.subjectAromatic Plantspt_PT
dc.subjectValidationpt_PT
dc.subjectComposição dos Alimentospt_PT
dc.titleUHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plantspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/COMPETE/PTDC%2FAGR-TEC%2F3366%2F2012/PT
oaire.citation.endPage450pt_PT
oaire.citation.issue2pt_PT
oaire.citation.startPage440pt_PT
oaire.citation.titleFood Analytical Methodspt_PT
oaire.citation.volume11pt_PT
oaire.fundingStreamCOMPETE
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPolítica editorial da revistapt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication48dca8ff-b5d3-4794-8901-2a10189a768f
relation.isProjectOfPublication.latestForDiscovery48dca8ff-b5d3-4794-8901-2a10189a768f

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