UHPLC-DAD Multi-Method for Determination of Phenolics in Aromatic Plants

Oliveira, Ana Sofia; Ribeiro-Santos, Regiane; Ramos, Fernando; Castilho, Maria Conceição; Sanches-Silva, Ana

http://hdl.handle.net/10400.18/6339

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Authors

Oliveira, Ana Sofia

Ribeiro-Santos, Regiane

Ramos, Fernando

Castilho, Maria Conceição

Sanches-Silva, Ana

Abstract(s)

The aim of this paper was to develop and validate a method of ultra high-performance liquid chromatography coupled with diode array detector (UHPLC-DAD), which allows quantifying simultaneously 19 phenolic compounds in aromatic plants. Twenty-one fresh and dried aromatic plants were extracted with methanol before their UHPLC-DAD analysis. The method was validated regarding specificity, working range, linearity, limit of detection (LOD) and quantification (LOQ), precision, and accuracy. The developed method allows detecting and quantifying the phenolic compounds at low concentrations (LOD < 0.15 μg/mL, except for carnosol and carnosic acid). The relative standard deviation obtained was lower than 5.73 and 8.75%, for repeatability and intermediate precision, respectively. The proposed method is straightforward and sensitive, with good precision and accuracy. Dried thyme presented the highest diversity of phenolic compounds and rosmarinic acid was present in most of the samples. This analytical tool is a valid alternative to conventional methods to quantify phenolic compounds in aromatic plants or in their extracts.