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The Use of Feathers from Racing Pigeons for Doping Control Purposes

2018-12-15Journal article Restricted access
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dc.contributor.authorMoreira, Fernando X.
dc.contributor.authorCarmo, Helena
dc.contributor.authorMelo, Armindo
dc.contributor.authorAndré, Maria B.
dc.contributor.authorSilva, Renata
dc.contributor.authorAzevedo, Zélia
dc.contributor.authorBastos, Maria L.
dc.contributor.authorde Pinho, Paula G.
dc.date.accessioned2019-03-27T12:22:21Z
dc.date.available2019-03-27T12:22:21Z
dc.date.issued2018-12-15
dc.description.abstractThe development of accurate and reliable analytical methodologies to detect the abuse of doping agents in sport animals is crucial to ensure their welfare, as well as to support continuing social acceptance of these sports. The detection of doping agents in racing pigeons is difficult, especially owing to the disadvantages and limitations of obtaining samples from conventional matrices. The present study aimed to develop and validate an analytical methodology combining a two-step extraction procedure (liquid-liquid extraction and solid-phase extraction) in feathers from racing pigeons with analysis by liquid chromatography tandem mass spectrometry (LC-MS-MS) that enabled the simultaneous detection of a beta-agonist drug (clenbuterol) and three corticosteroids (prednisolone, betamethasone and budesonide). The method was validated concerning linearity (with coefficients of determination always higher than 0.99), accuracy (87.3-112.4%), precision (repeatability and intermediate precision coefficient of variation (CV%) always below 15%), recovery (71.6-98.2%), limits of detection (0.24-0.52 ng/g) and quantification (0.79 and 0. 1.74 ng/g) and specificity. The applicability of the method was performed using feathers from pigeons administered orally with a daily dose of 0.075 mg of betamethasone. The drug was administered during 60 days and successive analyses of feathers were performed, at the end of the administration protocol and also after ceasing the oral administration of the drug, for a three weeks period.pt_PT
dc.description.sponsorshipThis work was supported by the European Union (European Regional Development Fund funds POCI/01/0145/FEDER/007728); and National Funds (Fundação para a Ciência e a Tecnologia and Ministério da Educação e Ciência) under the Partnership Agreement PT2020 UID/MULTI/04378/2013. The study is a result of the project NORTE-01-0145-FEDER-000024, supported by Norte Portugal Regional Operational Programme (NORTE, 2020), under the PORTUGAL 2020 Partnership Agreement (DESignBIOtecHealth— New Technologies for three Health Challenges of Modern Societies: Diabetes, Drug Abuse and Kidney Diseases), through the European Regional Development Fund (ERDF). A.M. wishes to thank the Fundação para a Ciência Tecnologia for grants SFRH/BPD/86898/2012. R.S. acknowledges Fundação para a Ciência e Tecnologia for her Post-doctoral Grant (SFRH/BPD/110201/2015).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Anal Toxicol. 2019 May 1;43(4):307-315. doi: 10.1093/jat/bky088. Epub 2018 Dec 15.pt_PT
dc.identifier.doi10.1093/jat/bky088pt_PT
dc.identifier.issn0146-4760
dc.identifier.urihttp://hdl.handle.net/10400.18/6329
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relationRISK ASSESSMENT OF DISINFECTION BY-PRODUCTS IN DRINKING WATER: OCCURRENCE, EVALUATION AND CONTROL
dc.relation.publisherversionhttps://academic.oup.com/jat/advance-article/doi/10.1093/jat/bky088/5248517pt_PT
dc.subjectDoping Controlpt_PT
dc.subjectRacing Pigeonspt_PT
dc.subjectFeatherspt_PT
dc.subjectMethodologies to Detectionpt_PT
dc.titleThe Use of Feathers from Racing Pigeons for Doping Control Purposespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleRISK ASSESSMENT OF DISINFECTION BY-PRODUCTS IN DRINKING WATER: OCCURRENCE, EVALUATION AND CONTROL
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04378%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F86898%2F2012/PT
oaire.citation.endPage9pt_PT
oaire.citation.startPage1pt_PT
oaire.citation.titleJournal of Analytical Toxicologypt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPolítica editorial da revistapt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication72797dcd-bef0-4686-8fd4-4ffc88f8cb5b
relation.isProjectOfPublication4f28927c-9902-4cbf-8328-358d9417daa1
relation.isProjectOfPublication.latestForDiscovery72797dcd-bef0-4686-8fd4-4ffc88f8cb5b

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