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Mycobacterium tuberculosis genetic diversity and drug resistance across Portuguese-speaking countries and CPLP-TB: a novel framework and surveillance tool for the Lusophone community

2019-04Conference object Open access
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dc.contributor.authorPerdigão, João
dc.contributor.authorSilva, Carla
dc.contributor.authorDiniz, Jaciara
dc.contributor.authorPereira, Catarina
dc.contributor.authorMachado, Diana
dc.contributor.authorRamos, Jorge
dc.contributor.authorSilva, Hugo
dc.contributor.authorAbilleira, Fernanda
dc.contributor.authorBrum, Clarice
dc.contributor.authorReis, Ana J.
dc.contributor.authorMacedo, Maíra
dc.contributor.authorScaini, João L.
dc.contributor.authorSilva, Ana B.
dc.contributor.authorEsteves, Leonardo
dc.contributor.authorMacedo, Rita
dc.contributor.authorMaltez, Fernando
dc.contributor.authorClemente, Sofia
dc.contributor.authorCoelho, Elizabeth
dc.contributor.authorViegas, Sofia
dc.contributor.authorRabna, Paulo
dc.contributor.authorRodrigues, Amabélia
dc.contributor.authorTaveira, Nuno
dc.contributor.authorJordão, Luísa
dc.contributor.authorKritski, Afrânio
dc.contributor.authorLapa e Silva, José
dc.contributor.authorMokrousov, Igor
dc.contributor.authorCouvin, David
dc.contributor.authorRastogi, Nalin
dc.contributor.authorCouto, Isabel
dc.contributor.authorPain, Arnab
dc.contributor.authorMcNerney, Ruth
dc.contributor.authorClark, Taane G.
dc.contributor.authorvon Groll, Andrea
dc.contributor.authorDalla-Costa, Elis R.
dc.contributor.authorRossetti, Maria Lúcia
dc.contributor.authorda Silva, Pedro E.A
dc.contributor.authorViveiros, Miguel
dc.contributor.authorPortugal, Isabel
dc.date.accessioned2020-05-18T16:36:16Z
dc.date.available2020-05-18T16:36:16Z
dc.date.issued2019-04
dc.description.abstractBackground: Tuberculosis (TB) remains a major health problem within the Community of Portuguese Language Speaking Countries (CPLP). Despite the marked variation in TB incidence across its member-states and continued human migratory flux between countries, a considerable gap in the knowledge on the Mycobacterium tuberculosis population structure and strain circulation between the countries still exists. Materials and Methods: To address this, we have assembled and analyzed the largest CPLP M. tuberculosis molecular and drug susceptibility dataset, comprised by a total of 1447 clinical isolates, including 423 multidrug-resistant isolates, from five CPLP countries. Genotyping analysis was carried out by 15/24 Mycobacterial Interspersed Repetitive Unit – Variable Number of Tandem Repeat (MIRU-VNTR) and Spoligotyping. Drug Susceptibility testing was performed using standardized BACTEC 960 MGIT methodology or through the resazurin microtiter assay (REMA). Results: The data herein presented reinforces Latin American and Mediterranean (LAM) strains as the hallmark of M. tuberculosis populational structure in the CPLP coupled with country-specific differential prevalence of minor clades. Moreover, using high-resolution typing by 24-loci MIRU-VNTR, six cross-border genetic clusters were detected, thus supporting recent clonal expansion across the Lusophone space. To make this data available to the scientific community and public health authorities we developed CPLP-TB (available at http://cplp-tb.ff.ulisboa.pt), an online database coupled with web-based tools for exploratory data analysis. Conclusions: As a public health tool, CPLP-TB is expected to contribute to improved knowledge on the M. tuberculosis population structure and strain circulation within the CPLP, thus supporting risk assessment of strain-specific trends.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/6694
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectMycobacterium Tuberculosispt_PT
dc.subjectGeneticspt_PT
dc.subjectSurveillancept_PT
dc.subjectCPLP-TBpt_PT
dc.subjectInfecções Respiratóriaspt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleMycobacterium tuberculosis genetic diversity and drug resistance across Portuguese-speaking countries and CPLP-TB: a novel framework and surveillance tool for the Lusophone communitypt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceAmsterdam, Netherlandpt_PT
oaire.citation.title19th European Congress of Clinical Microbiology and Infectious Diseases (ECCMID 2019), 13-16 April 2019pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT

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