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Analysis of the in vitro cytotoxicity and genotoxicity of cellulose nanomaterials in intestinal cells

2022-09Conference object Restricted access
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dc.contributor.authorVital, Nádia
dc.contributor.authorPinto, Fátima
dc.contributor.authorKranendonk, Michel
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2022-11-29T10:45:48Z
dc.date.available2022-11-29T10:45:48Z
dc.date.issued2022-09
dc.descriptionAbstract publicado em: Environ Mol Mutagen. 2022 Aug;63(Suppl 1):88. (Abstracts from the 13th International Conference on Environmental Mutagens and 53rd Annual Meeting of the Environmental Mutagenesis and Genomics Society). https://onlinelibrary.wiley.com/toc/10982280/2022/63/S1pt_PT
dc.description.abstractCellulose nanomaterials(CNMs) are recognized as promising bionanomaterials due to their natural and renewable source and attractive properties, with potential applications in multiple food-related products, as zero-calorie filler/thickener/stabilizer, or as substitutes of petroleum-based food packaging materials. These applications in food lead to human exposure through ingestion and their biopersistence raise concerns about their potential impact on the gastrointestinal tract. The present study aimed to investigate the in vitro cytotoxicity and genotoxicity of two types of micro/nanofibrillated celluloses(CMF/CNFs) using a human intestinal cell line. CMF and CNF were synthetized from industrial bleached Eucalyptus globulus kraft and their physicochemical properties were previously characterized. Following Caco-2 cells exposure to a concentration-range of CMF or CNF, the cytotoxicity was assessed by the MTT and clonogenic assays, while genotoxicity was assessed by the cytokinesis block micronucleus assay(CBMN). Outcomes indicated no cytotoxic or genotoxic effects upon exposure of Caco-2 cells to these CNMs for the tested concentration-range, suggesting their biocompatibility. This is the first study using the CBMN assay after Caco-2 cells exposure to CNMs. The results of ongoing studies using in vitro simulation of human digestion will allow a more comprehensive assessment of CNMs safety. By incrementing the knowledge on the cellular effects of novel CNMs in the human intestine, we expect to contribute to their safety assessment at an early-stage of their technological development towards the sustainable innovation in food technology.pt_PT
dc.description.sponsorshipFunded by FCT/MCTES through national funds (PTDC/SAU-PUB/29481/2017; PTDC/SAU-PUB/ 32587/2017) and (UIDB/00009/2020; UIDP/00009/2020); Nádia Vital holds a FCT/MCTES PhD Scholarship with the reference 2020.07168.BD.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8359
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.relationToxicological profile of cellulose nanomaterials in human gastrointestinal cells
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectCellulose Nanomaterialspt_PT
dc.subjectMicro/nanofibrillated Cellulosept_PT
dc.subjectCytotoxicitypt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectIntestinal Cellspt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleAnalysis of the in vitro cytotoxicity and genotoxicity of cellulose nanomaterials in intestinal cellspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleToxicological profile of cellulose nanomaterials in human gastrointestinal cells
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.07168.BD/PT
oaire.citation.conferencePlaceOttawa, Canadapt_PT
oaire.citation.title13th International Conference on Environmental Mutagens (ICEM) / 53rd Annual Meeting of the Environmental Mutagenesis and Genomics Society (EMGS), 28 aug - 1 sep 2022pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isProjectOfPublication10a38358-808e-4126-a323-58973bebbcce
relation.isProjectOfPublication0c494598-bc88-4e48-8032-cb9f4b4c0b0f
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