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Analysis of the in vitro cytotoxicity and genotoxicity of cellulose nanomaterials in intestinal cells
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Orientador(es)
Resumo(s)
Cellulose nanomaterials(CNMs) are recognized as promising bionanomaterials due to their natural and
renewable source and attractive properties, with potential applications in multiple food-related products, as
zero-calorie filler/thickener/stabilizer, or as substitutes of petroleum-based food packaging materials. These
applications in food lead to human exposure through ingestion and their biopersistence raise concerns about
their potential impact on the gastrointestinal tract.
The present study aimed to investigate the in vitro cytotoxicity and genotoxicity of two types of micro/nanofibrillated celluloses(CMF/CNFs) using a human intestinal cell line. CMF and CNF were synthetized
from industrial bleached Eucalyptus globulus kraft and their physicochemical properties were previously
characterized. Following Caco-2 cells exposure to a concentration-range of CMF or CNF, the cytotoxicity was
assessed by the MTT and clonogenic assays, while genotoxicity was assessed by the cytokinesis block
micronucleus assay(CBMN).
Outcomes indicated no cytotoxic or genotoxic effects upon exposure of Caco-2 cells to these CNMs for the
tested concentration-range, suggesting their biocompatibility. This is the first study using the CBMN assay after
Caco-2 cells exposure to CNMs. The results of ongoing studies using in vitro simulation of human digestion will
allow a more comprehensive assessment of CNMs safety. By incrementing the knowledge on the cellular effects of novel CNMs in the human intestine, we expect to contribute to their safety assessment at an early-stage of their technological development towards the sustainable innovation in food technology.
Descrição
Abstract publicado em: Environ Mol Mutagen. 2022 Aug;63(Suppl 1):88. (Abstracts from the 13th International Conference on Environmental Mutagens and 53rd Annual Meeting of the Environmental Mutagenesis and Genomics Society). https://onlinelibrary.wiley.com/toc/10982280/2022/63/S1
Palavras-chave
Environmental Genotoxicity Cellulose Nanomaterials Micro/nanofibrillated Cellulose Cytotoxicity Genotoxicity Intestinal Cells Genotoxicidade Ambiental