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Molecular heterogeneity of Rhipicephalus sanguineus sensu lato and screening for Ehrlichia canis in mainland Portugal

2018-09Journal article Restricted access
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dc.contributor.authorSantos, G.S.
dc.contributor.authorCouto, J.
dc.contributor.authorSilva-Perdosa, R.
dc.contributor.authorFerrolho, J.
dc.contributor.authorSantos, A.S.
dc.contributor.authorSantos-Silva, M.M.
dc.contributor.authorAntunes, S.
dc.contributor.authorDomingos, A.
dc.date.accessioned2019-02-18T11:27:11Z
dc.date.available2019-02-18T11:27:11Z
dc.date.issued2018-09
dc.description.abstractThe present study aimed to expand knowledge regarding the molecular characterization of R. sanguineus s.l. in Portugal and to screen for ticks naturally infected with E. canis. A total of 113 R. sanguineus s.l. were collected questing or attached to domestic and wild animals from the 18 administrative regions of mainland Portugal. All the ticks were analyzed for the 16S rRNA and the partial sequences obtained showed high genetic similarities with specimens belonging to the temperate lineage. These sequences revealed eight haplotypes (H1‒H8), with a genetic distance ranging from 0.3% to 1.4%. A convenience sample representing approximately 75% of all the R. sanguineus s.l. ticks collected was tested for the presence of E. canis by qPCR for the dsb gene. No ticks were found to be infected with this pathogen. Accordingly, further studies are required to determine the role of the R. sanguineus s.l. temperate lineage in E. canis maintenance and transmission, as well as to elucidate if a different R. sanguineus s.l. lineage or other tick species act as E. canis vectors for dogs in Portugal.pt_PT
dc.description.sponsorshipThis work was supported by Fundação para a Ciência e Tecnologia (FCT) for founds to GHTM (UID/Multi/04413/2013). This work was supported by FCT – Project PTDC/CVT-WEL/1807/2014.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationTicks Tick Borne Dis. 2018 Sep;9(6):1383-1390. doi: 10.1016/j.ttbdis.2018.06.002. Epub 2018 Jun 11pt_PT
dc.identifier.doi10.1016/j.ttbdis.2018.06.002. Epub 2018 Jun 11pt_PT
dc.identifier.other1877-959X/
dc.identifier.urihttp://hdl.handle.net/10400.18/5841
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://doi.org/10.1016/j.ttbdis.2018.06.002pt_PT
dc.subjectEhrlichia canispt_PT
dc.subjectRhipicephalus sanguineus s.l.pt_PT
dc.subjectTemperate Lineagept_PT
dc.subjectTick-borne Diseasespt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.subjectPortugalpt_PT
dc.titleMolecular heterogeneity of Rhipicephalus sanguineus sensu lato and screening for Ehrlichia canis in mainland Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04413%2F2013/PT
oaire.citation.endPage1390pt_PT
oaire.citation.issue6pt_PT
oaire.citation.startPage1383pt_PT
oaire.citation.titleTicks and Tick-borne Diseasespt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPolítica editorial da revistapt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationad39241e-5511-4aa7-975d-680cd5082c36
relation.isProjectOfPublication.latestForDiscoveryad39241e-5511-4aa7-975d-680cd5082c36

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