rTgOWP1-f, a specific biomarker for Toxoplasma gondii oocysts

Sousa, Susana; Almeida, André; Delgado, Lurdes; Conceição, Antónia; Marques, Cláudia; da Costa, José Manuel Correia; Castro, António

http://hdl.handle.net/10400.18/7624

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Authors

da Costa, José Manuel Correia

Castro, António

Abstract(s)

Toxoplasma gondii oocyst wall protein 1 (TgOWP1) integrates a family of seven proteins, consensually assumed as specific antigens of Toxoplasma gondii oocyst stage, located in the outer layer of the oocyst wall. Herein, we notice the expression of a recombinant antigen, rTgOWP1-f, derived from a fragment selected on basis of its structural homology with Plasmodium MSP1-19. Rabbit polyclonal antibodies anti-rTgOWP1-f evidence ability for specific identification of environmental T. gondii oocysts. We assume, rTgOWP1-f, as a possible biomarker of oocysts. In addition, we present findings supporting this vision, including the development of an immunodetection method for T. gondii oocysts identification.