SOP9 Buccal Cells Sampling - Partnership for the Assessment of the Risks from Chemicals

Louro, Henriqueta; Silva, Maria João

http://hdl.handle.net/10400.18/10536

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Authors

Louro, Henriqueta

Silva, Maria João

Abstract(s)

SOP9 Buccal Cells Sampling - presentation for PARC Training for Waste Management Survey / Partnership for the Assessment of the Risks from Chemicals. Aim of presentation: Provide the general procedure for the collection, storage, and transfer of buccal cell samples for micronucleus analysis in exfoliated buccal cells. The buccal micronucleus assay is a minimally invasive approach for measuring DNA damage, cell proliferation, cell differentiation and cell death in exfoliated buccal cells (Bolognesi et al., 2015). It offers a great opportunity to evaluate in a clear and precise way the appearance of genetic damage whether it is present as a consequence of occupational or environmental risk, being reliable, fast, relatively simple, cheap, and minimally invasive and causes no pain. This Standard Operating Procedure (SOP) for buccal cells sampling is intended to be used in the PARC waste management survey. The SOP provides the general procedure for the collection, storage, and transfer of buccal cell samples.