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Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications

2020-08-26Journal article Open access
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dc.contributor.authorGuerreiro, Adriana
dc.contributor.authorAndrade, Mariana
dc.contributor.authorMenezes, Carina
dc.contributor.authorVilarinho, Fernanda
dc.contributor.authorDias, Elsa
dc.date.accessioned2021-03-15T16:53:35Z
dc.date.available2021-03-15T16:53:35Z
dc.date.issued2020-08-26
dc.descriptionThe HEK293T cell line and reagents for cell culture were kindly offered by Rafaela Lacerda, Department of Human Genetics, National Health Institute, Lisbon, Portugal.pt_PT
dc.description.abstractAntioxidant compounds from cyanobacteria may constitute a natural alternative to current synthetic antioxidants, which contain preservatives and suspected toxicity. In this work, we evaluate the antioxidant potential of cyanobacterial strains of distinct species/genus isolated from freshwater (n = 6), soil (n = 1) and wastewater (n = 1) environments. Lyophilized biomass obtained from in-vitro cultures of those strains was extracted with ethanol and methanol. The antioxidant potential was evaluated by chemical (DPPH scavenging method, β-carotene bleaching assay, determination of total phenolic and total flavonoid compounds) and biological (H2O2-exposed HEK293T cell line model) approach. Some strains showed high yields of antioxidant activity by the DPPH assay (up to 10.7% IP/20.7 TE µg/mL) and by the β-carotene bleaching assay (up to 828.94 AAC), as well as significant content in phenolic (123.16 mg EAG/g DW) and flavonoid (900.60 mg EQR/g DW) compounds. Normalization of data in a “per cell” or “per cell volume” base might facilitate the comparison between strains. Additionally, most of the cyanobacterial extracts conferred some degree of protection to HEK293T cells against the H2O2-induced cytotoxicity. Freshwater Aphanizomenon gracile (LMECYA 009) and Aphanizomenon flos-aquae (LMECYA 088), terrestrial Nostoc (LMECYA 291) and wastewater Planktothrix mougeotii (LEGE 06224) seem to be promising strains for further investigation on cyanobacteria antioxidant potential.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationToxins (Basel). 2020 Aug 26;12(9):548. doi: 10.3390/toxins12090548pt_PT
dc.identifier.doi10.3390/toxins12090548pt_PT
dc.identifier.issn2072-6651
dc.identifier.urihttp://hdl.handle.net/10400.18/7501
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2072-6651/12/9/548pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCyanobacteriapt_PT
dc.subjectAntioxidant Potentialpt_PT
dc.subjectNatural Antioxidantspt_PT
dc.subjectDPPH Assaypt_PT
dc.subjectβ-carotene Bleaching Assaypt_PT
dc.subjectPhenolicpt_PT
dc.subjectFlavonoidspt_PT
dc.subjectHEK293T Cell Linept_PT
dc.subjectCytotoxicity Preventionpt_PT
dc.subjectSegurança Alimentarpt_PT
dc.subjectComposição dos Alimentospt_PT
dc.subjectToxicologia dos Alimentospt_PT
dc.subjectToxicologiapt_PT
dc.subjectAgentes Microbianos e Ambientept_PT
dc.titleAntioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applicationspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue9pt_PT
oaire.citation.startPage548pt_PT
oaire.citation.titleToxinspt_PT
oaire.citation.volume12pt_PT
rcaap.embargofctAcesso de acordo com página web do editor da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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