Antioxidant and Cytoprotective Properties of Cyanobacteria: Potential for Biotechnological Applications

Guerreiro, Adriana; Andrade, Mariana; Menezes, Carina; Vilarinho, Fernanda; Dias, Elsa

http://hdl.handle.net/10400.18/7501

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Abstract(s)

Antioxidant compounds from cyanobacteria may constitute a natural alternative to current synthetic antioxidants, which contain preservatives and suspected toxicity. In this work, we evaluate the antioxidant potential of cyanobacterial strains of distinct species/genus isolated from freshwater (n = 6), soil (n = 1) and wastewater (n = 1) environments. Lyophilized biomass obtained from in-vitro cultures of those strains was extracted with ethanol and methanol. The antioxidant potential was evaluated by chemical (DPPH scavenging method, β-carotene bleaching assay, determination of total phenolic and total flavonoid compounds) and biological (H2O2-exposed HEK293T cell line model) approach. Some strains showed high yields of antioxidant activity by the DPPH assay (up to 10.7% IP/20.7 TE µg/mL) and by the β-carotene bleaching assay (up to 828.94 AAC), as well as significant content in phenolic (123.16 mg EAG/g DW) and flavonoid (900.60 mg EQR/g DW) compounds. Normalization of data in a “per cell” or “per cell volume” base might facilitate the comparison between strains. Additionally, most of the cyanobacterial extracts conferred some degree of protection to HEK293T cells against the H2O2-induced cytotoxicity. Freshwater Aphanizomenon gracile (LMECYA 009) and Aphanizomenon flos-aquae (LMECYA 088), terrestrial Nostoc (LMECYA 291) and wastewater Planktothrix mougeotii (LEGE 06224) seem to be promising strains for further investigation on cyanobacteria antioxidant potential.

Description

The HEK293T cell line and reagents for cell culture were kindly offered by Rafaela Lacerda, Department of Human Genetics, National Health Institute, Lisbon, Portugal.

Keywords

Cyanobacteria Antioxidant Potential Natural Antioxidants DPPH Assay β-carotene Bleaching Assay Phenolic Flavonoids HEK293T Cell Line Cytotoxicity Prevention Segurança Alimentar Composição dos Alimentos Toxicologia dos Alimentos Toxicologia Agentes Microbianos e Ambiente