Publication
Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of helicobacter pylori clarithromycin resistance in gastric biopsy specimens
| dc.contributor.author | Cerqueira, L. | |
| dc.contributor.author | Fernandes, RM. | |
| dc.contributor.author | Ferreira, RM. | |
| dc.contributor.author | Oleastro, M. | |
| dc.contributor.author | Carneiro, F. | |
| dc.contributor.author | Brandão, C. | |
| dc.contributor.author | Pimentel-Nunes, P. | |
| dc.contributor.author | Dinis-Ribeiro, M. | |
| dc.contributor.author | Figueiredo, C. | |
| dc.contributor.author | Keevil, CW. | |
| dc.contributor.author | Vieira, MJ. | |
| dc.contributor.author | Azevedo, NF. | |
| dc.date.accessioned | 2013-05-24T17:05:46Z | |
| dc.date.available | 2013-05-24T17:05:46Z | |
| dc.date.issued | 2013-04-17 | |
| dc.description.abstract | Here, we evaluated a previously established peptide nucleic acid-fluorescence in situ hybridization (PNA-FISH) method as a new diagnostic test for Helicobacter pylori clarithromycin resistance detection in paraffin-embedded gastric biopsy specimens. Both a retrospective study and a prospective cohort study were conducted to evaluate the specificity and sensitivity of a PNA-FISH method to determine H. pylori clarithromycin resistance. In the retrospective study (n 30 patients), full agreement between PNA-FISH and PCR-sequencing was observed. Compared to the reference method (culture followed by Etest), the specificity and sensitivity of PNA-FISH were 90.9% (95% confidence interval [CI], 57.1% to 99.5%) and 84.2% (95% CI, 59.5% to 95.8%), respectively. In the prospective cohort (n 93 patients), 21 cases were positive by culture. For the patients harboring clarithromycin- resistant H. pylori, the method showed sensitivity of 80.0% (95% CI, 29.9% to 98.9%) and specificity of 93.8% (95% CI, 67.7% to 99.7%). These values likely represent underestimations, as some of the discrepant results corresponded to patients infected by more than one strain. PNA-FISH appears to be a simple, quick, and accurate method for detecting H. pylori clarithromycin resistance in paraffin-embedded biopsy specimens. It is also the only one of the methods assessed here that allows direct and specific visualization of this microorganism within the biopsy specimens, a characteristic that allowed the observation that cells of different H. pylori strains can subsist in very close proximity in the stomach. | por |
| dc.identifier.citation | J Clin Microbiol. 2013 Jun;51(6):1887-93. doi: 10.1128/JCM.00302-13. Epub 2013 Apr 17 | por |
| dc.identifier.issn | 0095-1137 | |
| dc.identifier.other | doi: 10.1128/JCM.00302-13 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/1585 | |
| dc.language.iso | eng | por |
| dc.peerreviewed | yes | por |
| dc.publisher | American Society for Microbiology | por |
| dc.relation | FCT (Bolsa de PhD SFRH/BD/38124/2007 e projeto PIC/IC/82815/2007 | por |
| dc.relation.publisherversion | http://jcm.asm.org/content/51/6/1887.full.pdf+html | por |
| dc.subject | Helicobacter pylori | por |
| dc.subject | Peptide Nucleic Acid-fluorescence in Situ Hybridization | por |
| dc.subject | Macrolides Resistance | por |
| dc.subject | Infecções Gastrointestinais | por |
| dc.title | Validation of a fluorescence in situ hybridization method using peptide nucleic acid probes for detection of helicobacter pylori clarithromycin resistance in gastric biopsy specimens | por |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.citation.endPage | 1893 | por |
| oaire.citation.startPage | 1887 | por |
| oaire.citation.title | Journal of Clinical Microbiology | por |
| oaire.citation.volume | 51(6) | por |
| rcaap.rights | openAccess | por |
| rcaap.type | article | por |