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Emergence of multidrug-resistant Mycobacterium tuberculosis of the Beijing lineage in Portugal and Guinea-Bissau: a snapshot of moving clones by whole-genome sequencing

2020-12Journal article Open access
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dc.contributor.authorPerdigão, João
dc.contributor.authorSilva, Carla
dc.contributor.authorMaltez, Fernando
dc.contributor.authorMachado, Diana
dc.contributor.authorMiranda, Anabela
dc.contributor.authorCouto, Isabel
dc.contributor.authorRabna, Paulo
dc.contributor.authorFlorez de Sessions, Paola
dc.contributor.authorPhelan, Jody
dc.contributor.authorPain, Arnab
dc.contributor.authorMcNerney, Ruth
dc.contributor.authorHibberd, Martin L.
dc.contributor.authorMokrousov, Igor
dc.contributor.authorClark, Taane G.
dc.contributor.authorViveiros, Miguel
dc.contributor.authorPortugal, Isabel
dc.date.accessioned2020-10-29T16:53:04Z
dc.date.available2020-10-29T16:53:04Z
dc.date.issued2020-12
dc.description.abstractThe Beijing genotype comprises a highly disseminated strain type that is frequently associated with multidrug resistant (MDR) tuberculosis (TB) and increased transmissibility but, countries such as Portugal and Guinea-Bissau fall outside the regions phylogeographically associated with this specific genotype. Nevertheless, recent data shows that this genotype might be gradually emerging in these two countries as an underlying cause of primary MDR-TB. Here, we describe the emergence of Mycobacterium tuberculosis Beijing strains associated with MDR-TB in Portugal and Guinea-Bissau demonstrating the presence of the well described superclusters 100-32 and 94-32 in Portugal and Guinea-Bissau, respectively. Genome-wide analysis and comparison with a global genomic dataset of M. tuberculosis Beijing strains, revealed the presence of two genomic clusters encompassing isolates from Portugal and Guinea-Bissau, GC1 (n = 121) and GC2 (n = 39), both of which bore SNP signatures compatible with the 100-32/B0/W148 and 94-32/Central Asia Outbreak clades, respectively. Moreover, GC2 encompasses a cross-border cluster between Portugal, Guinea-Bissau and Brazil thus supporting migration-associated introduction of MDR-TB and subsequent clonal expansion at the community-level. The comparison with global Beijing datasets demonstrates the global reach of the disease and its complex dissemination across multiple countries while in parallel there are clear microevolutionary trajectories towards extensively drug resistant TB.pt_PT
dc.description.sponsorshipThis work was supported by the Biotechnology and Biological Sciences Research Council [grant number BB/R013063/ 1]; Fundação para a Ciência e a Tecnologia [grant number UID/DTP/04138/2019,UID/Multi/04413/2013]; Medical Research Council [grant numbers MR/K000551/1, MR/ M01360X/1, MR/N010469/1, MR/R0209]; Russian Science Foundation [grant number 19-14-00013].pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationEmerg Microbes Infect. 2020 Dec;9(1):1342-1353. doi: 10.1080/22221751.2020.1774425. Epub 2020 Jun 15pt_PT
dc.identifier.doi10.1080/22221751.2020.1774425pt_PT
dc.identifier.issn2222-1751
dc.identifier.urihttp://hdl.handle.net/10400.18/7229
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherTaylor & Francispt_PT
dc.relation.publisherversionhttps://www.tandfonline.com/doi/full/10.1080/22221751.2020.1774425pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectBeijingpt_PT
dc.subjectMIRU-VNTRpt_PT
dc.subjectMtbC15-9 100-32pt_PT
dc.subjectMtbC15-9 94-32pt_PT
dc.subjectMycobacteriapt_PT
dc.subjectMigrationpt_PT
dc.subjectTuberculosispt_PT
dc.subjectInfecções Respiratóriaspt_PT
dc.subjectPortugalpt_PT
dc.subjectGuinea-Bissaupt_PT
dc.titleEmergence of multidrug-resistant Mycobacterium tuberculosis of the Beijing lineage in Portugal and Guinea-Bissau: a snapshot of moving clones by whole-genome sequencingpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FDTP%2F04138%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FDTP%2F04138%2F2013/PT (2019)
oaire.citation.endPage1353pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage1342pt_PT
oaire.citation.titleEmerging Microbes and Infectionspt_PT
oaire.citation.volume9pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication8b7dc2e1-c04d-4a11-8fa9-83ead9d8f256
relation.isProjectOfPublication0972f714-9fcc-4fca-ba3e-3c169e8aaf5b
relation.isProjectOfPublication.latestForDiscovery0972f714-9fcc-4fca-ba3e-3c169e8aaf5b

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