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Mycobacterium appelbergii sp. nov., a Novel Species Isolated from a Drinking Water Fountain in a Rural Community

2025-05-29Artigo científico Acesso aberto
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dc.contributor.authorRoxo, Inês Cravo
dc.contributor.authorAlarico, Susana
dc.contributor.authorFonseca, Ana
dc.contributor.authorMachado, Daniela
dc.contributor.authorMaranha, Ana
dc.contributor.authorTiago, Igor
dc.contributor.authorDuarte, Raquel
dc.contributor.authorEmpadinhas, Nuno
dc.date.accessioned2026-02-13T16:02:22Z
dc.date.available2026-02-13T16:02:22Z
dc.date.issued2025-05-29
dc.descriptionThis article belongs to the Topic Microbiota Diversity and Its Broader Biological Implications Across Human and Animal Health)
dc.description.abstractThree isolates of a novel, rapidly growing, non-pigmented Mycobacterium species were recovered from the water and runoff of a public fountain in a rural village in central Portugal, formerly used by the local population as a source of drinking water and not accessible to animals. High-quality draft genome sequencing, in silico DNA–DNA hybridization, and phylogenetic analyses confirmed that isolates 21AC1T, 21AC14, and 21AC21 represent a previously undescribed species within the genus Mycobacterium, forming a distinct phylogenetic lineage closely related to Mycobacterium wolinskyi, Mycobacterium goodii and Mycobacterium smegmatis. MALDI-TOF MS analysis of the type strain 21AC1T revealed a unique spectral profile. A comprehensive polyphasic characterization was performed, including chemotaxonomic analyses of fatty acid and mycolic acid composition, as well as an extensive biochemical characterization. Their susceptibility to 12 antimicrobials was also assessed. The identification and characterization of novel nontuberculous mycobacteria species are of increasing environmental and clinical relevance, as infections by these opportunistic pathogens are on the rise globally. Based on our findings, we propose that isolates 21AC1T, 21AC14, and 21AC21 represent a novel species, for which we propose the name Mycobacterium appelbergii sp. nov., with the type strain designated as 21AC1T (=BCCM/ITM 501212 = DSM 113570) and the additional two strains as 21AC14 (=BCCM/ITM 501447 = DSM 118402) and 21AC21 (=BCCM/ITM 501448 = DSM 118403).eng
dc.description.sponsorshipThis work was funded through the COMPETE 2020—Operational Programme for Competitiveness and Internationalisation and Portuguese national funds via FCT—Fundação para a Ciência e a Tecnologia, under projects PTDC/BIA-MIC/0122/2021 and projects UIDB/04539/2020, UIDP/04539/2020 and LA/P/0058/2020.
dc.identifier.citationMicroorganisms. 2025 May 29;13(6):1259. doi: 10.3390/microorganisms13061259
dc.identifier.doi10.3390/microorganisms13061259
dc.identifier.eissn2076-2607
dc.identifier.pmid40572149
dc.identifier.urihttp://hdl.handle.net/10400.18/10936
dc.language.isoeng
dc.peerreviewedyes
dc.publisherMDPI
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationCenter for Innovative Biomedicine and Biotechnology
dc.relationPTDC/BIA-MIC/0122/2021
dc.relationLA/P/0058/2020.
dc.relation.hasversionhttps://www.mdpi.com/2076-2607/13/6/1259
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectNontuberculous Mycobacteria
dc.subjectSpring Water
dc.subjectBiodiversity
dc.subjectFountain
dc.subjectGenomics
dc.subjectPhylogeny
dc.subjectChemotaxonomy
dc.subjectAgentes Microbianos e Ambiente
dc.titleMycobacterium appelbergii sp. nov., a Novel Species Isolated from a Drinking Water Fountain in a Rural Communityeng
dc.typejournal article
dcterms.referenceshttps://www.mdpi.com/article/10.3390/microorganisms13061259/s1
dspace.entity.typePublication
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardTitleCenter for Innovative Biomedicine and Biotechnology
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04539%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04539%2F2020/PT
oaire.citation.issue6
oaire.citation.startPage1259
oaire.citation.titleMicroorganisms
oaire.citation.volume13
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublication1ccb5f8f-0639-4328-8585-3d07f4854b0f
relation.isProjectOfPublicationbeb86cc2-5421-4df8-834f-1bbc09b43866
relation.isProjectOfPublication.latestForDiscovery1ccb5f8f-0639-4328-8585-3d07f4854b0f

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