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Phenotypic and Molecular Characterization of Salmonella 1,4,[5],12:i:- R-Type ASSuT Isolates from Humans, Animals, and Environment in Portugal, 2006-2011

2016-11Journal article Restricted access
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dc.contributor.authorSeixas, Rui
dc.contributor.authorSantos, Tânia Raquel
dc.contributor.authorMachado, Jorge
dc.contributor.authorTavares, Luís
dc.contributor.authorBernardo, Fernando
dc.contributor.authorSemedo-Lemsaddek, Teresa
dc.contributor.authorOliveira, Manuela
dc.date.accessioned2017-03-03T15:09:05Z
dc.date.available2017-03-03T15:09:05Z
dc.date.issued2016-11
dc.description.abstractThe increase in prevalence of Salmonella 1,4,[5],12:i:- related infections over the last few years has been considered a public health issue in many European countries, especially as this serovar may be associated with tetraresistance to ampicillin, streptomycin, sulfonamides, and tetracyclines (R-type ASSuT). Salmonella 1,4,[5],12:i:- isolates (n = 187) obtained by the Portuguese National Laboratory from different sources, including human clinical cases (n = 170), veterinary (n = 10), environmental (n = 6), and food samples (n = 1), were collected from 15 districts between 2006 and 2011. All isolates were serotyped using the slide agglutination method and results were confirmed by multiplex PCR for the monophasic variant. From the confirmed Salmonella 1,4,[5],12:i:-, R-type ASSuT isolates were selected by disc diffusion and minimal inhibitory concentration (MIC) determination for further characterization by pulsed-field gel electrophoresis restriction with XbaI, virulence genes determination by PCR, additional antimicrobial resistance profiling by disc diffusion, and epidemiological distribution evaluation. Out of the 187 serotyped isolates, 133 were confirmed as Salmonella 1,4,[5],12:i:- with a R-type ASSuT occurrence of 61.7%. Distribution among Portuguese districts showed a higher percentage of reported cases in coastal areas, in particular, in Porto (24.8%), Setúbal (13.5%), and Aveiro (12.8%), probably due to the higher population density. Clonality analysis revealed a high diversity of pulsotypes with the majority of human salmonellosis cases being attributed to sporadic events. All isolates harbored 14 out of the 18 virulence genes evaluated and 87.8% of the isolates showed all the resistance genes frequently associated with the European clone, blaTEM(+)sul2(+)straA-straB(+)tetB(+). This study shows that Salmonella 1,4,[5],12:i:- resistant isolates are widely distributed in Portugal. This may be related to a selective advantage offered by R-type ASSuT profile, the presence of multiple virulent features, including the ability to form biofilms, which along with a high diversity of pulsotypes may be responsible for the dissemination through the country.pt_PT
dc.description.sponsorshipThis study was conducted with the financial support of the ‘‘Centro de Investigac¸a˜o Interdisciplinar em Sanidade Animal, Faculdade de Medicina Veterina´ria da Universidade de Lisboa’’—Project UID/CVT/00276/2013 (funded by Foundation for Science and Technology—FCT). R.S. and T.R.S. hold a PhD fellowship (SFRH/BD/75836/2011 and SFRH/BD/100571/2014, respectively) from FCT, Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFoodborne Pathog Dis. 2016 Nov;13(11):633-641. Epub 2016 Oct 21.pt_PT
dc.identifier.doi10.1089/fpd.2016.2156pt_PT
dc.identifier.issn1535-3141
dc.identifier.urihttp://hdl.handle.net/10400.18/4446
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMary Ann Liebertpt_PT
dc.relation.publisherversionhttp://online.liebertpub.com/doi/pdf/10.1089/fpd.2016.2156pt_PT
dc.subjectSalmonellapt_PT
dc.subjectSeroverspt_PT
dc.subjectR-type ASSuTpt_PT
dc.subjectVirulence Factorspt_PT
dc.subjectPortugalpt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.titlePhenotypic and Molecular Characterization of Salmonella 1,4,[5],12:i:- R-Type ASSuT Isolates from Humans, Animals, and Environment in Portugal, 2006-2011pt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FCVT%2F00276%2F2013/PT
oaire.citation.endPage641pt_PT
oaire.citation.issue11pt_PT
oaire.citation.startPage633pt_PT
oaire.citation.titleFoodborne Pathogens and Diseasept_PT
oaire.citation.volume13pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication55874201-5b80-454f-84b5-d55e0fbd844a
relation.isProjectOfPublication.latestForDiscovery55874201-5b80-454f-84b5-d55e0fbd844a

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