Publicação
Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation
| dc.contributor.author | Ramos, Philippe E. | |
| dc.contributor.author | Abrunhosa, Luis | |
| dc.contributor.author | Pinheiro, Ana | |
| dc.contributor.author | Cerqueira, Miguel | |
| dc.contributor.author | Motta, Carla | |
| dc.contributor.author | Castanheira, Isabel | |
| dc.contributor.author | Chandra-Hioe, Maria V. | |
| dc.contributor.author | Arcot, Jayashree | |
| dc.contributor.author | Teixeira, José A. | |
| dc.contributor.author | Vicente, António A. | |
| dc.date.accessioned | 2017-03-06T17:01:36Z | |
| dc.date.embargo | 2025-10-24 | |
| dc.date.issued | 2016-10-29 | |
| dc.description.abstract | This study focused on the use of a new system, an alginate|Ɛ-poly-L-lysine| alginate|chitosan microcapsule (APACM), able to immobilize a folate-producing probiotic, Lactococcus lactis ssp. cremoris (LLC), which provides a new approach to the utilization of capsules and probiotics for in situ production of vitamins. LLC is able to produce 95.25±26 μg·L−1 of folate, during 10 h, andwas encapsulated in the APACM. APACMproved its capacity to protect LLC against the harsh conditions of a simulated digestion maintaining a viable concentration of 6 log CFU·mL−1of LLC. A nutrients exchange capacity test, was performed using Lactobacillus plantarum UM7, a high lactic acid producer was used here to avoid false negative results. The production and release of 2 g·L−1 of lactic acidwas achieved through encapsulation of L. plantarum, after 20 h. The adhesion of APACM to epithelial cells was also quantified, yielding 38% and 33% of capsules adhered to HT-29 cells and Caco-2 cells, respectively. | pt_PT |
| dc.description.sponsorship | The authors Philippe E. Ramos and Ana Pinheiro, are recipient of fellowships from the Fundacão para a Ciência e Tecnologia, POPHQREN and FSE (FCT, Portugal) through grants, SFRH/BD/80800/ 2012 and SFRH/BPD/101181/2014, respectively. The authors thank the FCT Strategic Project PEst-OE/EQB/LA0023/2013 and the project “BioInd—Biotechnology and Bioengineering for Improved Industrial and Agro-Food Processes”, ref. NORTE-07-0124-FEDER-000028 cofunded by the Programa Operacional Regional do Norte (ON.2–O Novo Norte), QREN, FEDER. | pt_PT |
| dc.description.version | info:eu-repo/semantics/publishedVersion | pt_PT |
| dc.identifier.citation | Food Res Int. 2016;90:25-32. doi:10.1016/j.foodres.2016.10.036 | pt_PT |
| dc.identifier.doi | http://dx.doi.org/10.1016/j.foodres.2016.10.036 | pt_PT |
| dc.identifier.issn | 0963-9969 | |
| dc.identifier.uri | http://hdl.handle.net/10400.18/4513 | |
| dc.language.iso | eng | pt_PT |
| dc.peerreviewed | yes | pt_PT |
| dc.publisher | Elsevier/Canadian Institute of Food Science and Technology (CIFST) | pt_PT |
| dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0963996916304781 | pt_PT |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | pt_PT |
| dc.subject | Probiotic | pt_PT |
| dc.subject | Immobilization | pt_PT |
| dc.subject | Mucoadhesive | pt_PT |
| dc.subject | Segurança Alimentar | pt_PT |
| dc.title | Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation | pt_PT |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/3599-PPCDT/PEst-OE%2FEQB%2FLA0023%2F2013/PT | |
| oaire.citation.endPage | 32 | pt_PT |
| oaire.citation.startPage | 25 | pt_PT |
| oaire.citation.title | Food Research International | pt_PT |
| oaire.citation.volume | 90 | pt_PT |
| oaire.fundingStream | 3599-PPCDT | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| rcaap.rights | embargoedAccess | pt_PT |
| rcaap.type | article | pt_PT |
| relation.isProjectOfPublication | cb0d8dbe-02b2-4383-8181-f93235775034 | |
| relation.isProjectOfPublication.latestForDiscovery | cb0d8dbe-02b2-4383-8181-f93235775034 |