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Publicação
Probiotic-loaded microcapsule system for human in situ folate production: Encapsulation and system validation
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Orientador(es)
Resumo(s)
This study focused on the use of a new system, an alginate|Ɛ-poly-L-lysine| alginate|chitosan microcapsule
(APACM), able to immobilize a folate-producing probiotic, Lactococcus lactis ssp. cremoris (LLC), which provides
a new approach to the utilization of capsules and probiotics for in situ production of vitamins. LLC is able to produce
95.25±26 μg·L−1 of folate, during 10 h, andwas encapsulated in the APACM. APACMproved its capacity to
protect LLC against the harsh conditions of a simulated digestion maintaining a viable concentration of
6 log CFU·mL−1of LLC. A nutrients exchange capacity test, was performed using Lactobacillus plantarum UM7,
a high lactic acid producer was used here to avoid false negative results. The production and release of 2 g·L−1
of lactic acidwas achieved through encapsulation of L. plantarum, after 20 h. The adhesion of APACM to epithelial
cells was also quantified, yielding 38% and 33% of capsules adhered to HT-29 cells and Caco-2 cells, respectively.
Descrição
Palavras-chave
Probiotic Immobilization Mucoadhesive Segurança Alimentar
Contexto Educativo
Citação
Food Res Int. 2016;90:25-32. doi:10.1016/j.foodres.2016.10.036
Editora
Elsevier/Canadian Institute of Food Science and Technology (CIFST)