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Investigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cells

2022-03Journal article Open access
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dc.contributor.authorVieira, Adriana
dc.contributor.authorVital, Nádia
dc.contributor.authorRolo, Dora
dc.contributor.authorRoque, Rossana
dc.contributor.authorGonçalves, Lídia M.
dc.contributor.authorBettencourt, Ana
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2022-11-24T12:09:18Z
dc.date.available2022-11-24T12:09:18Z
dc.date.issued2022-03
dc.description.abstractThe widespread use of titanium dioxide nanomaterials (TiO2 NMs) in food and consumer products such as toothpaste or food contact materials, suggests the relevance of human oral exposure to these nanomaterials (NMs) and raises the possibility of adverse effects in the gastrointestinal tract (GIT). We previously showed that the in vitro digestion of TiO2 NMs may increase their toxicity in intestinal cells. In this work, we analyzed the genotoxicity and the intracellular reactive oxygen species induction by physiologically relevant concentrations of three different TiO2 NMs (NM-102, NM-103 and NM-105) in Caco-2 and HT29-MTX-E12 intestinal cells, while considering the potential influence of the digestion process in the NMs' physiochemical characteristics. The results evidenced a DNA-damaging effect dependent on the NM, more relevant for the rutile/anatase NM-105, possibly due to its lower hydrodynamic size in the cells medium. In addition, the results of the micronucleus assay suggest effects on chromosomal integrity, an indicator of cancer risk, in the HT29-MTX-E12 cells, for all the tested TiO2 NMs, especially after the in vitro digestion. This work supports the evidence for concerns on the use of TiO2 NMs as a food additive, recently reported by EFSA, and for their use in applications in consumer products that may drive human exposure through ingestion.pt_PT
dc.description.sponsorshipThis work was funded by national funds through the FCT - Foundation for Science and Technology, I.P., under the project PTDC/SAU-PUB/29481/2017. Research co-funded by UIDB/00009/2020; UIDP/00009/2020 (Centre for Toxicogenomics and Human Health – ToxOmics, FCT- Foundation for Science and Technology). NV holds a FCT PhD Scholarship grant (2020.07168.BD). iMed.ULisboa (UIDB/04138/2020 and UIDP/04138/2020) principal investigator grants CEECIND/03143/2017 (L. M. Gonçalves).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFood Chem Toxicol. 2022 Mar;161:112841. doi: 10.1016/j.fct.2022.112841. Epub 2022 Jan 31.pt_PT
dc.identifier.doi10.1016/j.fct.2022.112841pt_PT
dc.identifier.issn0278-6915
dc.identifier.urihttp://hdl.handle.net/10400.18/8331
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.relationResearch Institute for Medicines
dc.relationResearch Institute for Medicines
dc.relationNot Available
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0278691522000382?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectNanomaterialspt_PT
dc.subjectGenotoxicitypt_PT
dc.subjectIngested Nanomaterialspt_PT
dc.subjectIn vitro Simulated Digestionpt_PT
dc.subjectIntestinal Epithelial Cellspt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectTitanium Dioxidept_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleInvestigation of the genotoxicity of digested titanium dioxide nanomaterials in human intestinal cellspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleResearch Institute for Medicines
oaire.awardTitleNot Available
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F29481%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04138%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/CEEC IND 2017/CEECIND%2F03143%2F2017%2FCP1476%2FCT0005/PT
oaire.citation.startPage112841pt_PT
oaire.citation.titleFood and Chemical Toxicologypt_PT
oaire.citation.volume161pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStreamCEEC IND 2017
person.familyNameSilva
person.familyNameLouro
person.givenNameMaria Joao
person.givenNameHenriqueta
person.identifier157627
person.identifier.ciencia-id7710-643D-97A3
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0002-6060-0716
person.identifier.orcid0000-0001-9744-7332
person.identifier.scopus-author-id55944437600
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
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project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
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