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An HPLC Procedure for the Quantification of Aloin in Latex and Gel from Aloe barbadensis Leaves

2016-11-24Journal article Restricted access
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dc.contributor.authorSánchez-Machado, D.I.
dc.contributor.authorLópez-Cervantes, J.
dc.contributor.authorMariscal-Domínguez, M.F.
dc.contributor.authorCruz-Flores, P.
dc.contributor.authorCampas-Baypoli, O.N.
dc.contributor.authorCantú-Soto, E.U.
dc.contributor.authorSanches-Silva, A.
dc.date.accessioned2017-03-02T15:26:52Z
dc.date.available2017-03-02T15:26:52Z
dc.date.issued2016-11-24
dc.description.abstractAloin is an anthraquinone-C-glycoside present in Aloe vera. This compound is extremely variable among different species and highly depends on the growing conditions of the plants. The quantification of aloin in different extraction preparations has been a frequent problem due to the high instability of the compound. The aim of the present study is to develop and validated an analytical method for aloin detection in fresh and dry samples of Aloe barbadensis gel and latex using high performance liquid chromatography coupled to a diode array detector (HPLC-DAD). Phosphate buffered saline (pH 3) was selected as the extraction solvent. The aloin was separated using a Zorbax Eclipse AAA column (4.6 × 150 mm) at 35°C, and water and acetonitrile were used as the mobile phase at a flow rate of 0.9 mL/min. The linearity was satisfactory with a correlation coefficient greater than 0.999. Under these conditions, the method precision (relative standard deviation) was 3.71% for FL, 4.41% for dry latex, 0.81% for fresh gel and 4.42% for dry gel samples. Aloe latex was determined to have a greater amount of aloin than aloe gel. The method validation was satisfactory and exhibited adequate linearity, repeatability and accuracy.pt_PT
dc.description.sponsorshipThis research was financed by project PROFAPI no. 2016-0025 from Instituto Tecnológico de Sonora.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Chromatogr Sci. 2017;55(3):251-7. Epub 2016 Nov 24.pt_PT
dc.identifier.doi10.1093/chromsci/bmw179pt_PT
dc.identifier.issn0021-9665
dc.identifier.urihttp://hdl.handle.net/10400.18/4402
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherOxford University Presspt_PT
dc.relation.publisherversionhttps://academic.oup.com/chromsci/article-abstract/55/3/251/2568883/An-HPLC-Procedure-for-the-Quantification-of-Aloin?redirectedFrom=fulltextpt_PT
dc.subjectAloinpt_PT
dc.subjectAloe verapt_PT
dc.subjectComposição de Alimentospt_PT
dc.titleAn HPLC Procedure for the Quantification of Aloin in Latex and Gel from Aloe barbadensis Leavespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage257pt_PT
oaire.citation.startPage251pt_PT
oaire.citation.titleJournal of Chromatographic Sciencept_PT
oaire.citation.volume55(3)pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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