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Deciphering the role of cyanobacteria in water resistome: Hypothesis justifying the antibiotic resistance (phenotype and genotype) in Planktothrix genus

2018-10-12Journal article Restricted access
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dc.contributor.authorDias, Elsa
dc.contributor.authorOliveira, Micaela
dc.contributor.authorManageiro, Vera
dc.contributor.authorVasconcelos, Vitor
dc.contributor.authorCaniça, Manuela
dc.date.accessioned2019-02-15T13:51:50Z
dc.date.available2020-10-12T00:30:11Z
dc.date.issued2018-10-12
dc.description.abstractThe importance of environmental microorganisms in the emergence and dissemination of antibiotic resistance is an undeniable fact. However, cyanobacteria are not seen yet as putative players in the dynamic of environmental resistome, despite their ubiquity in water environments, where they are exposed to antibiotic pollution and in straight contact with native and pathogenic bacteria harboring antibiotic resistance genes (ARGs). In this work we evaluated the susceptibility of 8 strains of Planktothrix agardhii (from surface freshwaters reservoirs) and 8 strains of Planktothrix mougeotii (from a wastewater treatment plant) to several classes of antibiotics, using a microplate dilution method previously described by us. We also search for ARGs in those strains by molecular methods. None of the 16 tested strains were susceptible to trimethoprim, nalidixic acid and norfloxacin, from 0.0015–1.6 mg/L, but all were susceptible to streptomycin, gentamicin, kanamycin, ceftazidime and ceftriaxone. The minimum inhibitory concentrations (MICs) ranged between 0.05–0.8 mg/L for the aminoglycosides and 0.4–1.6 mg/L for the two β‑lactams. Major differences were found in the susceptibility to amoxicillin and tetracycline, with P. agardhii being susceptible (MIC of 0.05 mg/L and 0.4 mg/L, respectively) and P. mougeotii not susceptible. These distinct responses might be due to differences between species. However, the lower susceptibility of wastewater strains suggests that antibiotic resistance phenotype of cyanobacteria is related with their habitat. The failure to detect acquired genes conferring resistance to trimethoprim/quinolones, strongly supports the hypothesis that cyanobacteria are intrinsically resistant to these antibiotics. Interestingly, we detected a class-1-type integron and a sul1 gene in 3 strains of both P. agardhii and P. mougeotii, which supports the possibility of cyanobacteria to acquire and transfer antibiotic resistance determinants. In conclusion, the identification of ARGs and related integrons, as well as the reduced susceptibility to some antibiotics, suggests that cyanobacteria may play a role on environmental resistome.pt_PT
dc.description.sponsorshipFundação para a Ciência e Tecnologia - Bolsas SFRH/BPD/77981/2011 e SFRH/BPD/77486/2011pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSci Total Environ. 2019 Feb 20;652:447-454. doi: 10.1016/j.scitotenv.2018.10.167. Epub 2018 Oct 12pt_PT
dc.identifier.doi10.1016/j.scitotenv.2018.10.167pt_PT
dc.identifier.issn0048-969
dc.identifier.urihttp://hdl.handle.net/10400.18/5826
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationEVOLUTIONARY POTENTIAL OF OXYIMINO-BETA-LACTAM RESISTANCE DETERMINANTS IN ANIMALS AND IDENTIFICATION OF NEW ENZYME-INHIBITORS FOR VETERINARY USE
dc.relation.publisherversionhttps://doi.org/10.1016/j.scitotenv.2018.10.167pt_PT
dc.subjectPlankthotrixpt_PT
dc.subjectCyanobacteriapt_PT
dc.subjectAntibiotic Resistancept_PT
dc.subjectFreshwaterpt_PT
dc.subjectWastewaterpt_PT
dc.subjectNOECpt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.subjectAgentes Microbianos e Ambientept_PT
dc.subjectÁgua e Solopt_PT
dc.titleDeciphering the role of cyanobacteria in water resistome: Hypothesis justifying the antibiotic resistance (phenotype and genotype) in Planktothrix genuspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEVOLUTIONARY POTENTIAL OF OXYIMINO-BETA-LACTAM RESISTANCE DETERMINANTS IN ANIMALS AND IDENTIFICATION OF NEW ENZYME-INHIBITORS FOR VETERINARY USE
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/SFRH/SFRH%2FBPD%2F77981%2F2011/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F77486%2F2011/PT
oaire.citation.endPage454pt_PT
oaire.citation.startPage447pt_PT
oaire.citation.titleScience of the Total Environmentpt_PT
oaire.citation.titleScience of the Total Environment
oaire.citation.volume652pt_PT
oaire.fundingStreamSFRH
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctPolítica editorial da revistapt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationc75babea-0e5d-4eb3-abd8-98c198e04ccd
relation.isProjectOfPublication3b63e7d9-d684-42ae-aff9-36815c617501
relation.isProjectOfPublication.latestForDiscoveryc75babea-0e5d-4eb3-abd8-98c198e04ccd

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