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Assessment of benzoquinones and mycotoxins in cereal flour samples from Portugal within SafeGrains project

2019-05Conference object Restricted access
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dc.contributor.authorAlvito, Paula
dc.contributor.authorHilƔrio, C
dc.contributor.authorBarros, G
dc.contributor.authorDuarte, S
dc.contributor.authorPires, M.J.
dc.contributor.authorMagro, A
dc.contributor.authorAssunĆ§Ć£o, R.
dc.contributor.authorFerreira, RB
dc.contributor.authorCarvalho, MO
dc.date.accessioned2020-05-15T10:33:57Z
dc.date.available2020-05-15T10:33:57Z
dc.date.issued2019-05
dc.description.abstractOne of the most relevant problems in stored grain is the contamination by insects and mycotoxin-producing fungi. The red flour beetle, Tribolium castaneum (Herbst) (Coleoptera, Tenebrionidae), one of the major key-pests of milled grain, is resistant to Beauveria bassiana infection, producing benzoquinone-containing defensive secretions, that reduce fungal germination and growth. It can be affirmed that the tenebrionid cuticular secretions have antifungal properties. For T. castaneum, p-benzoquinones such as methyl-1,4-benzoquinone (MBQ) and ethyl-1,4-benzoquinone (EBQ) are major components of defensive secretions used as repellents and irritants. SafeGrains project (PTDC/ASPā€PLA/28350/2017) intends to contribute to clarify the interaction among pest infestation and associated benzoquinones and fungus and produced mycotoxins. The present study aims to implement and validate two HPLC methodologies to determine benzoquinones and mycotoxins in cereal flour samples. In a first attempt, HPLC methods will be optimized and validated for cereal flour samples (linearity and working range, limits of detection (LOD) and quantification (LOQ) as well as recovery). Once attained the satisfactory analytical conditions for these determinations, the presence of both chemicals will be quantified in 5 flour samples from Portugal, contaminated with insects (T. castaneum) or fungi (mycotoxin producer as Aspergillus sp) or both. Results will be presented and discussed concerning its contents in benzoquinones and mycotoxins and possible interactive effects.pt_PT
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT), through the research unit ID/AGR/04129/2013-LEAF and by project PTDC/ASP-PLA/28350/2017 (SafeGrains) and FCT/MEC-FEDER, within the PT2020 Partnership Agreement and Compete 2020 through CESAM (UID/AMB/50017/2019).pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/6663
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectBenzoquinonespt_PT
dc.subjectMycotoxinspt_PT
dc.subjectCereal Flourpt_PT
dc.subjectSeguranƧa Alimentarpt_PT
dc.subjectToxicologiapt_PT
dc.subjectAvaliaĆ§Ć£o de Riscopt_PT
dc.subjectSaĆŗde Humanapt_PT
dc.titleAssessment of benzoquinones and mycotoxins in cereal flour samples from Portugal within SafeGrains projectpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAGR%2F04129%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F50017%2F2013/PT
oaire.citation.conferencePlaceLisboa, Portugalpt_PT
oaire.citation.title41st Mycotoxin Workshop, Lisbon School of Health Technology (Instituto PolitƩcnico de Lisboa)/ Society for Mycotoxin Research, 6-8 May 2019pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
project.funder.nameFundaĆ§Ć£o para a CiĆŖncia e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isProjectOfPublication1e51f4b5-8ae2-48e9-8b2c-3fff541b227e
relation.isProjectOfPublication1d7db62a-6440-4ccb-a5cc-c3f76c8585b3
relation.isProjectOfPublication.latestForDiscovery1d7db62a-6440-4ccb-a5cc-c3f76c8585b3

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