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Comet Assay

dc.contributor.authorCosta, Solange
dc.contributor.authorTeixeira, João Paulo
dc.date.accessioned2015-01-30T13:43:46Z
dc.date.available2015-01-30T13:43:46Z
dc.date.issued2014
dc.description.abstractComet Assay is a simple and sensitive approach to assess genotoxic damage in different cell types, using only a small number of cells. Over the last decades it has gained wide acceptance as an important tool in fundamental DNA damage and repair studies with applications ranging from molecular epidemiology to genetic toxicology. In short, after alkaline DNA unwinding, subsequent electrophoresis causes the relaxed and broken fragments of DNA to move toward the anode away from the nucleoid core, appearing like a ‘comet.’ The distribution of DNA within the comet relates to the extent of DNA damage present in the cells.
dc.identifier.citationIn: Wexler P. (Ed.). Encyclopedia of Toxicology. 3rd edition. vol 4. Elsevier Inc., Academic Press, pp. 1020-23. ISBN: 9780123864543por
dc.identifier.doi10.1016/B978-0-12-386454-3.01072-1
dc.identifier.isbn9780123864543
dc.identifier.urihttp://hdl.handle.net/10400.18/2727
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevier Inc., Academic Presspor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/B9780123864543010721
dc.subjectBiological Monitoringpor
dc.subjectBiomarkerspor
dc.subjectComet Assaypor
dc.subjectDNA Damagepor
dc.subjectDNA Repairpor
dc.subjectEnvironmental Exposurepor
dc.subjectGenotoxicitypor
dc.subjectHuman Studiespor
dc.subjectOccupational Exposurepor
dc.subjectOxidative Damagepor
dc.subjectRisk Assessmentpor
dc.subjectValidation Studiespor
dc.subjectAr e Saúde Ocupacionalpor
dc.subjectGenotoxidade Ambiental
dc.titleComet Assay
dc.typebook part
dspace.entity.typePublication
oaire.citation.endPage1023por
oaire.citation.startPage1020por
oaire.citation.titleEncyclopedia of Toxicology, 3rd edition vol 4por
oaire.citation.volume4por
rcaap.rightsembargoedAccesspor
rcaap.typebookPartpor

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