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Authors
Advisor(s)
Abstract(s)
Comet Assay is a simple and sensitive approach to assess genotoxic damage in different cell types, using only a small number of cells. Over the last decades it has gained wide acceptance as an important tool in fundamental DNA damage and repair studies with applications ranging from molecular epidemiology to genetic toxicology. In short, after alkaline DNA unwinding, subsequent electrophoresis causes the relaxed and broken fragments of DNA to move toward the anode away from the nucleoid core, appearing like a ‘comet.’ The distribution of DNA within the comet relates to the extent of DNA damage present in the cells.
Description
Keywords
Biological Monitoring Biomarkers Comet Assay DNA Damage DNA Repair Environmental Exposure Genotoxicity Human Studies Occupational Exposure Oxidative Damage Risk Assessment Validation Studies Ar e Saúde Ocupacional Genotoxidade Ambiental
Pedagogical Context
Citation
In: Wexler P. (Ed.). Encyclopedia of Toxicology. 3rd edition. vol 4. Elsevier Inc., Academic Press, pp. 1020-23. ISBN: 9780123864543
Publisher
Elsevier Inc., Academic Press