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Polyphenols as resistance modulators in Arcobacter butzleri

2019-07Journal article Restricted access
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dc.contributor.authorSousa, Vanessa
dc.contributor.authorLuís, Ângelo
dc.contributor.authorOleastro, Mónica
dc.contributor.authorDomingues, Fernanda
dc.contributor.authorFerreira, Susana
dc.date.accessioned2020-04-29T04:46:13Z
dc.date.available2020-04-29T04:46:13Z
dc.date.issued2019-07
dc.description.abstractArcobacter butzleri is an emerging human and animal pathogen for which an increased prevalence of resistance to antibiotics has been observed, and so alternative compounds to modulate resistance of A. butzleri are required. This work aims to study the potential use of several polyphenols as efflux pump inhibitors (EPIs) and to evaluate their interaction with antibiotics, in order to enhance antibiotic activity against A. butzleri. The minimum inhibitory concentration (MIC) of (-)-epicatechin, (+)-catechin, rutin, gallic acid, caffeic acid, chlorogenic acid, resveratrol, pterostilbene, and pinosylvin was determined, in absence and presence of four known EPIs. Subsequently, ethidium bromide accumulation in presence of subinhibitory concentrations of polyphenols was evaluated, and the synergistic potential of the compounds with antibiotics was assessed by checkerboard dilution test. Only stilbenes presented activity against A. butzleri, with MIC values ranging between 64 and 512 μg/mL. The MIC determination of the polyphenols in the presence of subinhibitory concentrations of known EPIs showed that efflux pumps play a role in the resistance to these compounds. Stilbenes also induced a higher intracellular accumulation of ethidium bromide, indicating that they may inhibit the activity of efflux pumps. Checkerboard assays showed that several combinations of polyphenol/antibiotic had an additive effect against A. butzleri. Overall, the results indicate that some polyphenols reduce A. butzleri resistance to antibiotics, suggesting the potential of stilbenes as EPIs. The potential of resveratrol and pinosylvin as resistance modulators was evidenced, insofar as these compounds can even revert antibiotic resistance. Therefore, the use of polyphenols as resistance modulators could be an alternative to overcome the decreasing susceptibility of A. butzleri to antibiotics.pt_PT
dc.description.sponsorshipSusana Ferreira was supported by a fellowship (SFRH/BPD/101959/2014) from Fundação para a Ciência e Tecnologia and co-financed by Fundo Social Europeu. This work was supported by FEDER funds through the POCI - COMPETE 2020 - Operational Programme Competitiveness and Internationalization in Axis I— Strengthening research, technological development, and innovation (Project POCI-01-0145-FEDER-007491) and National Funds by FCT - Foundation for Science and Technology (Project UID/Multi/00709/2013).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFolia Microbiol (Praha). 2019 Jul;64(4):547-554. doi: 10.1007/s12223-019-00678-3. Epub 2019 Jan 13pt_PT
dc.identifier.doi10.1007/s12223-019-00678-3pt_PT
dc.identifier.issn0015-5632
dc.identifier.urihttp://hdl.handle.net/10400.18/6548
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlag/ Institute of Microbiology, Academy of Sciences of the Czech Republicpt_PT
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s12223-019-00678-3pt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectArcobacterpt_PT
dc.subjectBacterial Proteinspt_PT
dc.subjectDrug Resistance, Bacterialpt_PT
dc.subjectGram-Negative Bacterial Infectionspt_PT
dc.subjectHumanspt_PT
dc.subjectMicrobial Sensitivity Testspt_PT
dc.subjectPolyphenolspt_PT
dc.subjectInfecções Gastrointestinaispt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titlePolyphenols as resistance modulators in Arcobacter butzleript_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F00709%2F2013/PT
oaire.citation.endPage554pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage547pt_PT
oaire.citation.titleFolia Microbiologicapt_PT
oaire.citation.volume64pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublicationf5e75317-d96b-4bfe-97b5-3912e4125430
relation.isProjectOfPublication.latestForDiscoveryf5e75317-d96b-4bfe-97b5-3912e4125430

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