Repository logo
 
Publication

Role of 13C-urea breath test in experimental model of Helicobacter pylori infection in mice

2011-08Journal article Restricted access
Simple item page
dc.contributor.authorSantos, António Mário
dc.contributor.authorLopes, Teresa
dc.contributor.authorOleastro, Mónica
dc.contributor.authorChaves, Paula
dc.contributor.authorCordeiro, Rita
dc.contributor.authorFerreira, Maria
dc.contributor.authorPereira, Teresa
dc.contributor.authorMachado, Jorge
dc.contributor.authorGuerreiro, António Sousa
dc.date.accessioned2012-01-12T16:28:56Z
dc.date.available2012-01-12T16:28:56Z
dc.date.issued2011-08
dc.description.abstractBACKGROUND: Animal models have been widely used to study Helicobacter pylori infection. Evaluation of H. pylori infection status following experimental inoculation of mice usually requires euthanasia. The (13) C-urea breath test ((13) C-UBT) is both sensitive and specific for detection of H. pylori in humans. Thus, it would be very useful to have such a test with the same accuracy for the follow-up of this infection in animal models of gastric infection. Accordingly, the purpose of this study was to develop and evaluate a (13) C-UBT method for following the course of H. pylori infection in a mouse model. MATERIAL AND METHODS: A total of 50 female C57BL/6 mice were gavaged three times with either 10(8) colony-forming units of H. pylori (n=29) or saline solution only (n=21). After 2 months of infection, mice were fasted for 14 hours and (13) C-UBT was performed using 300 μg of (13) C-urea. The mice were killed, and the stomach was removed and processed for immunohistochemistry and PCR. RESULTS: The optimal time for breath sample collection in mice was found to be 15 minutes. The (13) C-UBT cutoff was set at 3.0‰ δPDB. Using PCR as the gold standard, the sensitivity of (13) C-UBT and immunohistochemistry was 96.6 and 72.4%, respectively, while the specificity was 85.7 and 95.2%, respectively. CONCLUSIONS: (13) C-UBT was shown to be a reliable method for the detection of H. pylori infection in C57BL/6 mice and was even more accurate than immunohistochemistry. The use of (13) C-UBT in the mouse model of H. pylori infection can be very useful to detect the bacterium without the need to kill the animals in long-term time course studies.por
dc.identifier.citationHelicobacter. 2011 Aug;16(4):320-6. Epub 2011 Jul 18por
dc.identifier.issn1083-4389
dc.identifier.otherdoi: 10.1111/j.1523-5378.2011.00847.x
dc.identifier.urihttp://hdl.handle.net/10400.18/370
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherBlackwell Publishing Ltapor
dc.relationFCT PT DC/SAU-OSM/66323/2006.por
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1111/j.1523-5378.2011.00847.x/abstractpor
dc.subject13C-UBTpor
dc.subjectMicepor
dc.subjectH. pyloripor
dc.subjectInfecções Gastrointestinaispor
dc.titleRole of 13C-urea breath test in experimental model of Helicobacter pylori infection in micepor
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage326por
oaire.citation.startPage320por
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Role of 13C-Urea Breath Test in Experimental Model of_2011.pdf
Size:
289.39 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DDI - Artigos em revistas internacionais