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Role of 13C-urea breath test in experimental model of Helicobacter pylori infection in mice
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Resumo(s)
BACKGROUND: Animal models have been widely used to study Helicobacter pylori
infection. Evaluation of H. pylori infection status following experimental
inoculation of mice usually requires euthanasia. The (13) C-urea breath test
((13) C-UBT) is both sensitive and specific for detection of H. pylori in humans.
Thus, it would be very useful to have such a test with the same accuracy for the
follow-up of this infection in animal models of gastric infection. Accordingly,
the purpose of this study was to develop and evaluate a (13) C-UBT method for
following the course of H. pylori infection in a mouse model.
MATERIAL AND METHODS: A total of 50 female C57BL/6 mice were gavaged three times
with either 10(8) colony-forming units of H. pylori (n=29) or saline solution
only (n=21). After 2 months of infection, mice were fasted for 14 hours and (13)
C-UBT was performed using 300 μg of (13) C-urea. The mice were killed, and the
stomach was removed and processed for immunohistochemistry and PCR.
RESULTS: The optimal time for breath sample collection in mice was found to be 15
minutes. The (13) C-UBT cutoff was set at 3.0‰ δPDB. Using PCR as the gold
standard, the sensitivity of (13) C-UBT and immunohistochemistry was 96.6 and
72.4%, respectively, while the specificity was 85.7 and 95.2%, respectively.
CONCLUSIONS: (13) C-UBT was shown to be a reliable method for the detection of H.
pylori infection in C57BL/6 mice and was even more accurate than
immunohistochemistry. The use of (13) C-UBT in the mouse model of H. pylori
infection can be very useful to detect the bacterium without the need to kill the
animals in long-term time course studies.
Descrição
Palavras-chave
13C-UBT Mice H. pylori Infecções Gastrointestinais
Contexto Educativo
Citação
Helicobacter. 2011 Aug;16(4):320-6. Epub 2011 Jul 18