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Potent cationic antimicrobial peptides against Mycobacterium tuberculosis in vitro

2019-12Journal article Restricted access
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dc.contributor.authorSilva, Sara
dc.contributor.authorSantos-Silva, Anabela
dc.contributor.authorda Costa, José Manuel Correia
dc.contributor.authorVale, Nuno
dc.date.accessioned2020-05-06T15:22:15Z
dc.date.available2020-05-06T15:22:15Z
dc.date.issued2019-12
dc.description.abstractBackground: Tuberculosis (TB) is known to be one of the 10 causes of global death by infectious agents. The increasing numbers of multiple antibiotic resistance (MDR-TB) and cases of extensive resistance to antibiotics (XDR-TB) have led to the development of new and effective TB therapy. Cationic antimicrobial peptides (CAMPs) have emerged in the research as a safe and effective treatment against a variable range of bacterial and fungi pathogens, including Mycobacterium tuberculosis (M. tuberculosis). Method: This study developed a new CAMP coupled with cinnamic acid derivatives, and studied the antimicrobial activity against clinical isolates of M. tuberculosis (H37Rv) and MDR-TB. Results: All modified CAMPs showed enhanced activity against both M. tuberculosis strains and were capable of disrupting heavy clumping of mycobacteria in culture. In addition, all modified CAMPs were able to substantially inhibit the intracellular growth of both strains at low concentrations. Conclusions: The characteristic proprieties of cinnamic acid+CAMP(n) successfully inhibited the growth of both clinical isolates M. tuberculosis and MDR-TB in vitro and have, for now, promising use as a drug adjuvant due to their effect on mycobacteria growth.pt_PT
dc.description.abstractHighlights: The conjugation of cationic peptides with cinnamic acid derivates enhanced antimicrobial activity; All modified cationic antimicrobial peptides (CAMPs) presented an increased antimicrobial activity against Mycobacterium tuberculosis; Microscopy visualisation demonstrated that modified CAMPs were able to inhibit cellular growth; These modified CAMPs presented antimicrobial activity against resistant strains of Mycobacterium tuberculosis.pt_PT
dc.description.sponsorshipThis work was financed by FEDER - Fundo Europeu de Desenvolvimento Regional funds through the COMPETE 2020 - Operacional Programme for Competitiveness and Internationali- sation (POCI), Portugal 2020, and by Portuguese funds through FCT - Fundação para a Ciência e a Tecnologia, in the framework of the project ‘Institute for Research and Innovation in Health Sciences’ (POCI-01-0145-FEDER-007274). This work was also funded by FCT and FEDER (European Union), through project IF/00092/2014/CP1255/CT0004. NV thanks FCT by IF position, DQB-Faculty of Sciences from University of Porto, for support in peptide synthesis, Fundação Manuel António da Mota (FMAM, Portugal) and Pfizer Portugal by support Nuno Vale Lab., and Dr Alexandra Fraga/Prof. Jorge Pedrosa from ICVS-U.Minho for cytotoxicity assay. SS thanks FCT and the Medicines and Pharmaceutical Innovation (i3DU) Doctoral Programme (i3DUPhD) for PhD grant with ref. PD/BD/135458/2017. JMCC thanks FCT for Pest-OE/AGR/UI0211/2011 and Strategic Project UI211. The contents of this report are solely the responsibility of the authors and do not necessarily represent the official views of the FCT, FMAM or Pfizer Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationJ Glob Antimicrob Resist. 2019 Dec;19:132-135. doi: 10.1016/j.jgar.2019.04.018. Epub 2019 May 30pt_PT
dc.identifier.doi10.1016/j.jgar.2019.04.018pt_PT
dc.identifier.issn2213-7165
dc.identifier.urihttp://hdl.handle.net/10400.18/6603
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/abs/pii/S2213716519301079?via%3Dihubpt_PT
dc.subjectTuberculosispt_PT
dc.subjectAntimicrobial Activitypt_PT
dc.subjectCationic Antimicrobial Peptides (CAMP)pt_PT
dc.subjectCinnamic Acidpt_PT
dc.subjectMycobacterium Tuberculosispt_PT
dc.subjectResistant Strainpt_PT
dc.subjectInfecções Respiratóriaspt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titlePotent cationic antimicrobial peptides against Mycobacterium tuberculosis in vitropt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage135pt_PT
oaire.citation.startPage132pt_PT
oaire.citation.titleJournal of Global Antimicrobial Resistancept_PT
oaire.citation.volume19pt_PT
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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