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Getting under the birds’ skin: tissue tropism of Borrelia burgdorferi s.l. in naturally and experimentally infected avian hosts

2019-10-15Journal article Restricted access
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dc.contributor.authorNorte, Ana Cláudia
dc.contributor.authorLopes de Carvalho, Isabel
dc.contributor.authorNúncio, Maria Sofia
dc.contributor.authorAraújo, Pedro Miguel
dc.contributor.authorMatthysen, Erik
dc.contributor.authorAlbino Ramos, Jaime
dc.contributor.authorSprong, Hein
dc.contributor.authorHeylen, Dieter
dc.date.accessioned2020-05-08T10:53:19Z
dc.date.available2020-05-08T10:53:19Z
dc.date.issued2019-10-15
dc.description.abstractWild birds are frequently exposed to the zoonotic tick-borne bacteria Borrelia burgdorferi sensu lato (s.l.), and some bird species act as reservoirs for some Borrelia genospecies. Studying the tropism of Borrelia in the host, how it is sequestered in different organs, and whether it is maintained in circulation and/or in the host's skin is important to understand pathogenicity, infectivity to vector ticks and reservoir competency.We evaluated tissue dissemination of Borrelia in blackbirds (Turdus merula) and great tits (Parus major), naturally and experimentally infected with Borrelia genospecies from enzootic foci. We collected both minimally invasive biological samples (feathers, skin biopsies and blood) and skin, joint, brain and visceral tissues from necropsied birds. Infectiousness of the host was evaluated through xenodiagnoses and infection rates in fed and moulted ticks. Skin biopsies were the most reliable method for assessing avian hosts' Borrelia infectiousness, which was supported by the agreement of infection status results obtained from the analysis of chin and lore skin samples from necropsied birds and of their xenodiagnostic ticks, including a significant correlation between the estimated concentration of Borrelia genome copies in the skin and the Borrelia infection rate in the xenodiagnostic ticks. This confirms a dermatropism of Borrelia garinii, B. valaisiana and B. turdi in its avian hosts. However, time elapsed from exposure to Borrelia and interaction between host species and Borrelia genospecies may affect the reliability of skin biopsies. The blood was not useful to assess infectiousness of birds, even during the period of expected maximum spirochetaemia. From the tissues sampled (foot joint, liver, spleen, heart, kidney, gut and brain), Borrelia was detected only in the gut, which could be related with infection mode, genospecies competition, genospecies-specific seasonality and/or excretion processes.pt_PT
dc.description.sponsorshipThis study received financial support from Fundação para a Ciência e a Tecnologia by the strategic program of MARE (MARE - UID/MAR/04292/2019), the fellowship to Ana Cláudia Norte (SFRH/BPD/108197/2015) and from the Portuguese National Institute of Health Doutor Ricardo Jorge. Dieter Heylen is funded by the Marie Sklodowska-Curie Actions (EU-Horizon 2020, Individual Global Fellowship, project no. 799609), the Fund for Scientific Research – Flanders (FWO) and the Bill & Melinda Gates Foundation.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationMicrob Ecol. 2020 Apr;79(3):756-769. doi: 10.1007/s00248-019-01442-3. Epub 2019 Oct 15.pt_PT
dc.identifier.doi10.1007/s00248-019-01442-3pt_PT
dc.identifier.issn0095-3628
dc.identifier.urihttp://hdl.handle.net/10400.18/6614
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherSpringer Verlagpt_PT
dc.relationEffects of co-infections on the emergence of an avian disease Mycoplasma gallisepticum
dc.relation.publisherversionhttps://link.springer.com/article/10.1007/s00248-019-01442-3pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAvian Reservoir Hostspt_PT
dc.subjectLyme borreliosispt_PT
dc.subjectMicroorganism Tropismpt_PT
dc.subjectSkin Biopsiespt_PT
dc.subjectTick-borne Pathogenspt_PT
dc.subjectInfecções Sistémicas e Zoonosespt_PT
dc.titleGetting under the birds’ skin: tissue tropism of Borrelia burgdorferi s.l. in naturally and experimentally infected avian hostspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleEffects of co-infections on the emergence of an avian disease Mycoplasma gallisepticum
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMAR%2F04292%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/EC/H2020/799609/EU
oaire.citation.endPage769pt_PT
oaire.citation.issue3pt_PT
oaire.citation.startPage756pt_PT
oaire.citation.titleMicrobial Ecologypt_PT
oaire.citation.volume79pt_PT
oaire.fundingStream5876
oaire.fundingStreamH2020
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100008530
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameEuropean Commission
rcaap.embargofctDe acordo com política editorial da revista.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication0dd4a5cd-2c09-4417-9190-3de42fbc5f7c
relation.isProjectOfPublication8db1d0b4-c269-4fce-92c5-5e1f5b01a179
relation.isProjectOfPublication.latestForDiscovery8db1d0b4-c269-4fce-92c5-5e1f5b01a179

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