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Occurrence of extended-spectrum β-lactamases among isolates of Salmonella enterica subsp. enterica from food-producing animals and food products, in Portugal

2013-10-15Journal article Restricted access
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dc.contributor.authorClemente, Lurdes
dc.contributor.authorManageiro, Vera
dc.contributor.authorFerreira, Eugénia
dc.contributor.authorJones-Dias, Daniela
dc.contributor.authorCorreia, Ivone
dc.contributor.authorThemudo, Patrícia
dc.contributor.authorAlbuquerque, Teresa
dc.contributor.authorCaniça, Manuela
dc.date.accessioned2017-02-17T11:09:50Z
dc.date.available2017-10-16T00:30:14Z
dc.date.issued2013-10-15
dc.description.abstractA total of 1120 Salmonella spp. isolates, recovered from poultry, swine and food products of animal origin (bovine, swine and poultry) over the period of 2009-2011, were investigated in order to determine their serotype, susceptibility to a panel of eleven antimicrobials (A, ampicillin; Ct, cefotaxime; Cp, ciprofloxacin; Tm, trimethoprim; Su, sulfamethoxazole; C, chloramphenicol; S, streptomycin; G, gentamicin; T, tetracycline; NA, nalidixic acid; Fl, florfenicol), and the presence of resistance determinants of extended-spectrum cephalosporins. Overall, Salmonella Enteritidis was the most common serotype in all three animal species. In 618 isolates of poultry, 32.8% comprised S. Enteritidis, 18.3% Salmonella Havana and 16.5% Salmonella Mbandaka; in 101 isolates of pigs, 21.8% comprised Salmonella Rissen and Salmonella Typhimurium, 10.9% Salmonella Derby and Salmonella London. Salmonella I 4,[5],12:i:- was the most common serotype recovered from pork and beef food products comprising 32.6% and 30% of isolates respectively, followed by S. Rissen (26% and 24%) and S. Typhimurium (18.2% and 19%), respectively. In poultry products, S. Enteritidis was the most frequent serotype (62.7%), followed by S. Mbandaka (10.2%) and S. Derby (8.5%). Susceptibility profiles differed according to the origin of the isolates. Five multidrug resistant isolates (0.45%) were further characterized as extended-spectrum β-lactamase (ESBL) producers. Polymerase chain reaction and sequencing of the amplicons confirmed the presence of bla(CTX-M-1) (n = 2), bla(CTX-M-14) (n = 1), bla(CTX-M-15) (n = 1) and bla(CTX-M-32) (n = 1); bla(SHV-12) and bla(TEM-1) genes were also detected in two isolates of S. I 4,[5],12:i:-. Four isolates, two S. Havana and two S. I 4,[5],12:i:-, carried class 1 integrons and in three, two S. I 4,[5],12:i:- and one S. Havana, ISEcp1 was identified associated to bla(CTX-M-1), bla(CTX-M-32) and bla(CTX-M-14) genes. Additionally, in one S. I 4,[5],12:i:- isolate, orf477 was identified linked to bla(CTX-M-32). No plasmid mediated quinolone resistance-encoding genes were detected. Here, we report for the first time the presence of bla(CTX-M) genes in Salmonella enterica subsp enterica isolates recovered from poultry and food products of swine origin, in Portugal.pt_PT
dc.description.sponsorshipV. Manageiro and D. Jones-Dias were supported by grants SFRH/BPD/77486/2011 and SFRH/BD/80001/2011, respectively, from Fundação para a Ciência e Tecnologia, Lisbon, Portugalpt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationInt J Food Microbiol. 2013 Oct 15;167(2):221-8. doi: 10.1016/j.ijfoodmicro.2013.08.009. Epub 2013 Aug 18pt_PT
dc.identifier.doi10.1016/j.ijfoodmicro.2013.08.009pt_PT
dc.identifier.issn0168-1605
dc.identifier.urihttp://hdl.handle.net/10400.18/4242
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevier/pt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0168160513003838pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectBacterial Proteinspt_PT
dc.subjectCattlept_PT
dc.subjectCephalosporin Resistancept_PT
dc.subjectDrug Resistance, Multiple, Bacterialpt_PT
dc.subjectMeatpt_PT
dc.subjectMicrobial Sensitivity Testspt_PT
dc.subjectPlasmidspt_PT
dc.subjectPolymerase Chain Reactionpt_PT
dc.subjectPortugalpt_PT
dc.subjectPoultrypt_PT
dc.subjectSalmonella entericapt_PT
dc.subjectSerotypingpt_PT
dc.subjectSwinept_PT
dc.subjectbeta-Lactamasespt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleOccurrence of extended-spectrum β-lactamases among isolates of Salmonella enterica subsp. enterica from food-producing animals and food products, in Portugalpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage228pt_PT
oaire.citation.startPage221pt_PT
oaire.citation.titleInternational Journal of Food Microbiologypt_PT
oaire.citation.volume167(2)pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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