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Genetic diversity, antibiotic resistance and biofilm-forming ability of Arcobacter butzleri isolated from poultry and environment from a Portuguese slaughterhouse

2013-03-01Journal article Restricted access
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dc.contributor.authorFerreira, Susana
dc.contributor.authorFraqueza, Maria João
dc.contributor.authorQueiroz, João
dc.contributor.authorDomingues, Fernanda
dc.contributor.authorOleastro, Mónica
dc.date.accessioned2013-04-15T11:40:42Z
dc.date.available2013-04-15T11:40:42Z
dc.date.issued2013-03-01
dc.description.abstractThe genus Arcobacter is an emerging pathogen associated with several clinical symptoms. This genus is widely distributed and has been isolated from environmental, animal, food and human samples, where poultry is considered the major source. In this study, forty three Arcobacter butzleri strains isolated from poultry and environment of a Portuguese slaughterhouse, were characterized by pulsed field gel electrophoresis (PFGE) and assessed for antimicrobial susceptibility and ability to form biofilms. PFGE patterns obtained using restriction enzymes SmaI and SacII revealed high genetic diversity, with 32 distinct PFGE patterns. Most of A. butzleri isolates presented multiple antimicrobial resistance, exhibiting four different resistance profiles. All 43 isolates were susceptible to gentamicin and 2.3% were resistant to chloramphenicol, in contrast to twenty four (55.8%) that were resistant to ciprofloxacin. Among 36 selected isolates, 26 strains presented biofilmforming ability, which was dependent on the atmosphere and initial inoculum density. Overall, the results showed that A. butzleri displays a high genetic diversity, and presents resistance to several antibiotics, which together with its biofilm formation ability may represent a potential hazard for foodborne infections and a considerable risk for human health.por
dc.identifier.citationInt J Food Microbiol. 2013 Mar 1;162(1):82-8. doi: 10.1016/j.ijfoodmicro.2013.01.003. Epub 2013 Jan 11por
dc.identifier.issn0168-1605
dc.identifier.otherdoi: 10.1016/j.ijfoodmicro.2013.01.003
dc.identifier.urihttp://hdl.handle.net/10400.18/1547
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherElsevierpor
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0168160513000135por
dc.subjectArcobacter Butzleripor
dc.subjectPoultrypor
dc.subjectPulsed Field Gel Electrophoresispor
dc.subjectAntimicrobial Resistancepor
dc.subjectBiofilmpor
dc.subjectInfecções Gastrointestinaispor
dc.titleGenetic diversity, antibiotic resistance and biofilm-forming ability of Arcobacter butzleri isolated from poultry and environment from a Portuguese slaughterhousepor
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876-PPCDTI/PTDC%2FAGR-ALI%2F121876%2F2010/PT
oaire.citation.endPage88por
oaire.citation.startPage82por
oaire.citation.titleInternational Journal of Food Microbiologypor
oaire.citation.volume162(1)por
oaire.fundingStream5876-PPCDTI
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspor
rcaap.typearticlepor
relation.isProjectOfPublication94aacdaa-3f70-497d-894e-10c4e62733c7
relation.isProjectOfPublication.latestForDiscovery94aacdaa-3f70-497d-894e-10c4e62733c7

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