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Genetic diversity, antibiotic resistance and biofilm-forming ability of Arcobacter butzleri isolated from poultry and environment from a Portuguese slaughterhouse
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Orientador(es)
Resumo(s)
The genus Arcobacter is an emerging pathogen associated with several clinical symptoms. This genus is widely
distributed and has been isolated from environmental, animal, food and human samples, where poultry is considered
the major source. In this study, forty three Arcobacter butzleri strains isolated from poultry and environment
of a Portuguese slaughterhouse, were characterized by pulsed field gel electrophoresis (PFGE) and
assessed for antimicrobial susceptibility and ability to form biofilms. PFGE patterns obtained using restriction
enzymes SmaI and SacII revealed high genetic diversity, with 32 distinct PFGE patterns. Most of A. butzleri isolates
presented multiple antimicrobial resistance, exhibiting four different resistance profiles. All 43 isolates
were susceptible to gentamicin and 2.3% were resistant to chloramphenicol, in contrast to twenty four
(55.8%) that were resistant to ciprofloxacin. Among 36 selected isolates, 26 strains presented biofilmforming
ability, which was dependent on the atmosphere and initial inoculum density.
Overall, the results showed that A. butzleri displays a high genetic diversity, and presents resistance to several
antibiotics, which together with its biofilm formation ability may represent a potential hazard for foodborne
infections and a considerable risk for human health.
Descrição
Palavras-chave
Arcobacter Butzleri Poultry Pulsed Field Gel Electrophoresis Antimicrobial Resistance Biofilm Infecções Gastrointestinais
Contexto Educativo
Citação
Int J Food Microbiol. 2013 Mar 1;162(1):82-8. doi: 10.1016/j.ijfoodmicro.2013.01.003. Epub 2013 Jan 11