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Differentially expressed microRNAs in human bronchial epithelial cells after exposure to nanofibrillar, microfibrillar or nanocrystalline cellulose

2022-09Conference object Restricted access
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dc.contributor.authorVentura, Célia
dc.contributor.authorVieira, Luís
dc.contributor.authorSilva, Catarina
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSilva, Maria João
dc.date.accessioned2022-11-28T11:42:11Z
dc.date.available2022-11-28T11:42:11Z
dc.date.issued2022-09
dc.descriptionToxicology Letters 368:S23pt_PT
dc.descriptionAbstracts publicado em: Toxicology Letters. 2022;368(Suppl 1):S23. (Abstracts of the XVIth International Congress of Toxicology, ICT 2022). https://ict-2022.elsevierdigitaledition.com/pt_PT
dc.description.abstractIn recent years, there has been a growing interest in nanocellulose, aninnovative engineered nanomaterial with physicochemical propertiesthat give it an enormous potential for use in awide varietyof industrialand biomedical applications. This expanding use is raising concern sabout its potential effects on human health after occupational,environmental or consumer exposure. To date, several studies aboutthe potential nanocellulose toxicity have been performed, mainly invitro, some indicating its biocompatibility, others suggesting geno-toxic or inflammatory effects, depending on the nanocellulose specificphysicochemical properties [1]. Nevertheless, to our knowledge, nostudy has addressed nanocellulose epigenotoxicity by analyzing itseffects at the microRNA expression level. Thus, this study aimed atidentifying the differentially expressed microRNAs (DEmiRNAs) in human bronchial epithelial cells (BEAS-2B cells), after 24 h exposure tothree different types of nanocellulose, two fibrillar (CNF and CMF;cellulose nanofibrils and cellulose microfibrils) and one crystalline(CNC; cellulose nanocrystals) derived fromEucaliptus globuluskraftpulp. For this purpose, microRNAs extracted from exposed and non-exposed cells were sequenced on a NextSeq 550 (Illumina). DEmiRNAswere obtained using sRNAtoolbox, and only DEmiRNAs identified atleast by two different methods were considered for further analysis.The results showed that both CNF and CMF did not change microRNAexpression on BEAS-2B cells, as they did not induce any statisticallysignificant (FDR⍰0.05) DEmiRNA as compared to non-exposed cells.By contrast, CNC induced the over- and under-expression of 22 and 30microRNAs, respectively. The ongoing bioinformatics study about theBEAS-2B cellular pathways that are enriched with these DEmiRNAswill give mechanistic insights that might help predicting its toxico-logical outcomes. Moreover, the profile of DEmiRNAs identified afterexposure to CNC will be further investigated in order to explore itspotential use as an effect biomarker for human biomonitoring studies.pt_PT
dc.description.sponsorshipPortuguese Foundation for Science and Technology (FCT/MCTES), through national funds (PIDDAC) under the project ToxApp4NanoCELFI (PTDC/SAU-PUB/32587/2017), and projects UIDB/00009)/2020 and UIDP/0009/2020 (ToxOmics).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/8336
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.relationA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
dc.relationCentre for Toxicogenomics and Human Health
dc.relationCentre for Toxicogenomics and Human Health
dc.subjectMicroRNAspt_PT
dc.subjectBronchial Epithelial Cellspt_PT
dc.subjectCellulose Nanofibrilspt_PT
dc.subjectCellulose Microfibrilspt_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.titleDifferentially expressed microRNAs in human bronchial epithelial cells after exposure to nanofibrillar, microfibrillar or nanocrystalline cellulosept_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardTitleA predictive toxicology approach to characterize potential respiratory effects of functionalized nanocellulose fibres in a co-culture system
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardTitleCentre for Toxicogenomics and Human Health
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FSAU-PUB%2F32587%2F2017/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F00009%2F2020/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F00009%2F2020/PT
oaire.citation.conferencePlaceMaastricht, The Netherlandspt_PT
oaire.citation.titleXVIth International Congress of Toxicology (ICT) 'Uniting in Toxicology, 18-21 September 2022pt_PT
oaire.fundingStream3599-PPCDT
oaire.fundingStream6817 - DCRRNI ID
oaire.fundingStream6817 - DCRRNI ID
person.familyNameVentura
person.familyNameLouro
person.familyNameSilva
person.givenNameCélia
person.givenNameHenriqueta
person.givenNameMaria Joao
person.identifier2056460
person.identifier157627
person.identifier.ciencia-id6116-89BA-C617
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.orcid0000-0002-0637-2222
person.identifier.orcid0000-0001-9744-7332
person.identifier.orcid0000-0002-6060-0716
person.identifier.scopus-author-id6507971479
person.identifier.scopus-author-id55944437600
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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