Differentially expressed microRNAs in human bronchial epithelial cells after exposure to nanofibrillar, microfibrillar or nanocrystalline cellulose

Ventura, Célia; Vieira, Luís; Silva, Catarina; Louro, Henriqueta; Silva, Maria João

http://hdl.handle.net/10400.18/8336

Use this identifier to reference this record.

Name:	Description:	Size:	Format:
VenturaC_ICT2022_nano.pdf		1.86 MB	Adobe PDF	Download
Toxicology Letters-s23.pdf		763.45 KB	Adobe PDF	Download

Send Feedback

Authors

Abstract(s)

In recent years, there has been a growing interest in nanocellulose, aninnovative engineered nanomaterial with physicochemical propertiesthat give it an enormous potential for use in awide varietyof industrialand biomedical applications. This expanding use is raising concern sabout its potential effects on human health after occupational,environmental or consumer exposure. To date, several studies aboutthe potential nanocellulose toxicity have been performed, mainly invitro, some indicating its biocompatibility, others suggesting geno-toxic or inflammatory effects, depending on the nanocellulose specificphysicochemical properties [1]. Nevertheless, to our knowledge, nostudy has addressed nanocellulose epigenotoxicity by analyzing itseffects at the microRNA expression level. Thus, this study aimed atidentifying the differentially expressed microRNAs (DEmiRNAs) in human bronchial epithelial cells (BEAS-2B cells), after 24 h exposure tothree different types of nanocellulose, two fibrillar (CNF and CMF;cellulose nanofibrils and cellulose microfibrils) and one crystalline(CNC; cellulose nanocrystals) derived fromEucaliptus globuluskraftpulp. For this purpose, microRNAs extracted from exposed and non-exposed cells were sequenced on a NextSeq 550 (Illumina). DEmiRNAswere obtained using sRNAtoolbox, and only DEmiRNAs identified atleast by two different methods were considered for further analysis.The results showed that both CNF and CMF did not change microRNAexpression on BEAS-2B cells, as they did not induce any statisticallysignificant (FDR⍰0.05) DEmiRNA as compared to non-exposed cells.By contrast, CNC induced the over- and under-expression of 22 and 30microRNAs, respectively. The ongoing bioinformatics study about theBEAS-2B cellular pathways that are enriched with these DEmiRNAswill give mechanistic insights that might help predicting its toxico-logical outcomes. Moreover, the profile of DEmiRNAs identified afterexposure to CNC will be further investigated in order to explore itspotential use as an effect biomarker for human biomonitoring studies.

Description

Toxicology Letters 368:S23
Abstracts publicado em: Toxicology Letters. 2022;368(Suppl 1):S23. (Abstracts of the XVIth International Congress of Toxicology, ICT 2022). https://ict-2022.elsevierdigitaledition.com/