Logo do repositório
 
Publicação

Impact of nanocelluloses on genome-wide DNA methylation pattern of human pulmonary and intestinal cells

2025-09-22Documento de conferência Acesso restrito
Ver registo simples
datacite.subject.fosCiências Médicas::Outras Ciências Médicas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorVentura, Célia
dc.contributor.authorVital, Nádia
dc.contributor.authorValente, Ana
dc.contributor.authorVieira, Luís
dc.contributor.authorLouro, Henriqueta
dc.contributor.authorSilva, Maria João
dc.date.accessioned2026-03-24T12:09:23Z
dc.date.available2026-03-24T12:09:23Z
dc.date.issued2025-09-22
dc.description.abstractObjective: Nanocellulose is an innovative nanomaterial with interesting physicochemical properties for several industrial and biomedical applications and its safety for human health must be ensured. This study aimed to identify DNA methylation changes in human pulmonary and intestinal cells after exposure to two fibrillar celluloses with different physicochemical properties, both derived from Eucaliptus globulus. Their cellular effects were investigated in silico by functional pathway and gene ontology (GO) analysis. Methods: We applied Reduced Representation Bisulfite Sequencing to analyze the methylation differences in DNA CpG-rich regions from human bronchial (BEAS-2B) and intestinal (Caco-2) cells exposed for 24h to 14.3 µg/mL of cellulose nanofibrils (CNF) or microfibrils (CMF) versus non-exposed ones. A bioinformatics pipeline was implemented for identifying differentially methylated genes (DMGs), functional pathways, and GO associations. Results: CNF and CMF exposure resulted in 11 and 14 DMGs, respectively, in BEAS-2B cells, 6 being common to both nanocelluloses. In Caco-2 cells, 36 and 31 DMGs were identified, sharing 12 DMGs. No DMGs were shared between these cell lines. Hypomethylation predominated in BEAS-2B cells, and hypermethylation in Caco-2 cells. In BEAS-2B cells, both nanocelluloses affected similar pathways and GO terms (e.g., regulation of DNA replication, damage repair and senescence, telomere maintenance, and D-glucose transport). In Caco-2 cells, both CNF and CMF enriched, for instance, signal transduction, glycosylation, and cytoskeletal dynamics. Each nanocellulose type also affected other different pathways and terms. Conclusions: Nanocellulose may have a wide impact on the metabolism and survival of pulmonary and intestinal cells through several regulatory pathways, which depend on nanocellulose physicochemical properties. Cell type also influences the outcome, suggesting tissue-specific effects. These findings highlight the relevance of DNA methylation in nanotoxicology, providing insights into underlying molecular mechanisms of action. Keywords: gene ontology; nanomaterial; pathway analysis; RRBSeng
dc.description.sponsorshipToxicological profile of cellulose nanomaterials in human gastrointestinal cells
dc.identifier.urihttp://hdl.handle.net/10400.18/11293
dc.language.isoeng
dc.peerreviewedn/a
dc.relationToxicological profile of cellulose nanomaterials in human gastrointestinal cells
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDNA Methylation
dc.subjectNanocellulose
dc.subjectPARC
dc.subjectEnvironmental Genotoxicity
dc.subjectGenotoxicidade Ambiental
dc.titleImpact of nanocelluloses on genome-wide DNA methylation pattern of human pulmonary and intestinal cellseng
dc.typeconference object
dspace.entity.typePublication
oaire.awardNumber2020.07168.BD
oaire.awardTitleToxicological profile of cellulose nanomaterials in human gastrointestinal cells
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//2020.07168.BD/PT
oaire.citation.conferenceDate2025-09
oaire.citation.conferencePlaceÉvora, Portugal
oaire.citation.title13th International Symposium on Biological Monitoring in Occupational and Environmental Health (ISBM), 9-12 September 2025
oaire.versionhttp://purl.org/coar/version/c_b1a7d7d4d402bcce
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublicationc0104504-d9a5-4fcb-a93d-8916a1c75df4
relation.isProjectOfPublication.latestForDiscoveryc0104504-d9a5-4fcb-a93d-8916a1c75df4

Ficheiros

Principais
A mostrar 1 - 2 de 2
Miniatura indisponível
Nome:
PPT_OP167_Methylation.pptx
Tamanho:
18.02 MB
Formato:
Microsoft Powerpoint XML
Ver/Abrir
Miniatura
Nome:
Impact of nanocelluloses on genome-wide DNA methylation pattern of human pulmonary and intestinal cells.pdf
Tamanho:
1.92 MB
Formato:
Adobe Portable Document Format
Ver/Abrir
Licença
A mostrar 1 - 1 de 1
Miniatura indisponível
Nome:
license.txt
Tamanho:
4.03 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Ver/Abrir

Coleções

DGH - Apresentações orais em encontros nacionais