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INFOGEST static in vitro simulation of gastrointestinal food digestion

2019-04Journal article Restricted access
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dc.contributor.authorBrodkorb, André
dc.contributor.authorEgger, Lotti
dc.contributor.authorAlminger, Marie
dc.contributor.authorAlvito, Paula
dc.contributor.authorAssunção, Ricardo
dc.contributor.authorBallance, Simon
dc.contributor.authorBohn, Torsten
dc.contributor.authorBourlieu-Lacanal, Claire
dc.contributor.authorBoutrou, Rachel
dc.contributor.authorCarrière, Frédéric
dc.contributor.authorClemente, Alfonso
dc.contributor.authorCorredig, Milena
dc.contributor.authorDupont, Didier
dc.contributor.authorDufour, Claire
dc.contributor.authorEdwards, Cathrina
dc.contributor.authorGolding, Matt
dc.contributor.authorKarakaya, Sibel
dc.contributor.authorKirkhus, Bente
dc.contributor.authorLe Feunteun, Steven
dc.contributor.authorLesmes, Uri
dc.contributor.authorMacierzanka, Adam
dc.contributor.authorMackie, Alan R.
dc.contributor.authorMartins, Carla
dc.contributor.authorMarze, Sébastien
dc.contributor.authorMcClements, David Julian
dc.contributor.authorMénard, Olivia
dc.contributor.authorMinekus, Mans
dc.contributor.authorPortmann, Reto
dc.contributor.authorSantos, Cláudia N.
dc.contributor.authorSouchon, Isabelle
dc.contributor.authorSingh, R. Paul
dc.contributor.authorVegarud, Gerd E.
dc.contributor.authorWickham, Martin S.J.
dc.contributor.authorWeitschies, Werner
dc.contributor.authorRecio, Isidra
dc.date.accessioned2019-07-10T14:16:19Z
dc.date.available2019-07-10T14:16:19Z
dc.date.issued2019-04
dc.description.abstractDeveloping a mechanistic understanding of the impact of food structure and composition on human health has increasingly involved simulating digestion in the upper gastrointestinal tract. These simulations have used a wide range of different conditions that often have very little physiological relevance, and this impedes the meaningful comparison of results. The standardized protocol presented here is based on an international consensus developed by the COST INFOGEST network. The method is designed to be used with standard laboratory equipment and requires limited experience to encourage a wide range of researchers to adopt it. It is a static digestion method that uses constant ratios of meal to digestive fluids and a constant pH for each step of digestion. This makes the method simple to use but not suitable for simulating digestion kinetics. Using this method, food samples are subjected to sequential oral, gastric and intestinal digestion while parameters such as electrolytes, enzymes, bile, dilution, pH and time of digestion are based on available physiological data. This amended and improved digestion method (INFOGEST 2.0) avoids challenges associated with the original method, such as the inclusion of the oral phase and the use of gastric lipase. The method can be used to assess the endpoints resulting from digestion of foods by analyzing the digestion products (e.g., peptides/amino acids, fatty acids, simple sugars) and evaluating the release of micronutrients from the food matrix. The whole protocol can be completed in ~7 d, including ~5 d required for the determination of enzyme activities.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationNat Protoc. 2019 Apr;14(4):991-1014. doi: 10.1038/s41596-018-0119-1. Epub 2019 Mar 18pt_PT
dc.identifier.doi10.1038/s41596-018-0119-1pt_PT
dc.identifier.issn1754-2189
dc.identifier.urihttp://hdl.handle.net/10400.18/6441
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Researchpt_PT
dc.relation.publisherversionhttps://www.nature.com/articles/s41596-018-0119-1pt_PT
dc.subjectAmino Acidspt_PT
dc.subjectBilept_PT
dc.subjectBiomimetic Materialspt_PT
dc.subjectDigestionpt_PT
dc.subjectEatingpt_PT
dc.subjectEnzyme Assayspt_PT
dc.subjectFatty Acidspt_PT
dc.subjectFoodpt_PT
dc.subjectFood Ingredientspt_PT
dc.subjectGastric Juicept_PT
dc.subjectHumanspt_PT
dc.subjectHydrogen-Ion Concentrationpt_PT
dc.subjectHydrolysispt_PT
dc.subjectIntestinespt_PT
dc.subjectMouthpt_PT
dc.subjectOligosaccharidespt_PT
dc.subjectPeptide Fragmentspt_PT
dc.subjectSalivapt_PT
dc.subjectStomachpt_PT
dc.subjectModels, Biologicalpt_PT
dc.subjectAvaliação do Impacte em Saúdept_PT
dc.subjectComposição dos Alimentospt_PT
dc.titleINFOGEST static in vitro simulation of gastrointestinal food digestionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage1014pt_PT
oaire.citation.issue4pt_PT
oaire.citation.startPage991pt_PT
oaire.citation.titleNature Protocolspt_PT
oaire.citation.volume14pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT

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