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Genomic structure and insertion sites of Helicobacter pylori prophages from various geographical origins

2017-02-16Journal article Open access
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dc.contributor.authorVale, Filipa F.
dc.contributor.authorNunes, Alexandra
dc.contributor.authorOleastro, Mónica
dc.contributor.authorGomes, João P.
dc.contributor.authorSampaio, Daniel A.
dc.contributor.authorRocha, Raquel
dc.contributor.authorVítor, Jorge M. B.
dc.contributor.authorEngstrand, Lars
dc.contributor.authorPascoe, Ben
dc.contributor.authorBerthenet, Elvire
dc.contributor.authorSheppard, Samuel K.
dc.contributor.authorHitchings, Matthew D.
dc.contributor.authorMégraud, Francis
dc.contributor.authorVadivelu, Jamuna
dc.contributor.authorLehours, Philippe
dc.date.accessioned2018-03-06T15:13:47Z
dc.date.available2018-03-06T15:13:47Z
dc.date.issued2017-02-16
dc.description.abstractHelicobacter pylori genetic diversity is known to be influenced by mobile genomic elements. Here we focused on prophages, the least characterized mobile elements of H. pylori. We present the full genomic sequences, insertion sites and phylogenetic analysis of 28 prophages found in H. pylori isolates from patients of distinct disease types, ranging from gastritis to gastric cancer, and geographic origins, covering most continents. The genome sizes of these prophages range from 22.6-33.0 Kbp, consisting of 27-39 open reading frames. A 36.6% GC was found in prophages in contrast to 39% in H. pylori genome. Remarkably a conserved integration site was found in over 50% of the cases. Nearly 40% of the prophages harbored insertion sequences (IS) previously described in H. pylori. Tandem repeats were frequently found in the intergenic region between the prophage at the 3' end and the bacterial gene. Furthermore, prophage genomes present a robust phylogeographic pattern, revealing four distinct clusters: one African, one Asian and two European prophage populations. Evidence of recombination was detected within the genome of some prophages, resulting in genome mosaics composed by different populations, which may yield additional H. pylori phenotypes.pt_PT
dc.description.sponsorshipThis work was supported by the the Fundação para a Ciência e a Tecnologia (FCT) project grant PTDC/EBBEBI/119860/2010 and by the University of Malaya-Ministry of Education (UM-MoE) High Impact Research (HIR) Grant UM.C/HIR/MOHE/13/5 (h-50001-00-A000033). F.F.V. is recipient of a postdoctoral fellowship from FCT (SFRH/BPD/95125/2013).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSci Rep. 2017 Feb 16;7:42471. doi: 10.1038/srep42471.pt_PT
dc.identifier.doi10.1038/srep42471pt_PT
dc.identifier.issn2045-2322 (print)
dc.identifier.issn2045-2322 (online)
dc.identifier.urihttp://hdl.handle.net/10400.18/5212
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherNature Publishing Grouppt_PT
dc.relationHelicobacter pylori phages: translating an underestimated phenomenon into a therapeutic application
dc.relationMicro-lysis: studing Helicobacter pylori phages for phage therapy
dc.relation.publisherversionhttps://www.nature.com/articles/srep42471pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHelicobacter pyloript_PT
dc.subjectProphagespt_PT
dc.subjectInsertion Sitespt_PT
dc.subjectGeographypt_PT
dc.subjectGastrointestinal Infections
dc.subjectInfecções Gastrointestinais
dc.titleGenomic structure and insertion sites of Helicobacter pylori prophages from various geographical originspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleHelicobacter pylori phages: translating an underestimated phenomenon into a therapeutic application
oaire.awardTitleMicro-lysis: studing Helicobacter pylori phages for phage therapy
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FEBB-EBI%2F119860%2F2010/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBPD%2F95125%2F2013/PT
oaire.citation.startPage42471pt_PT
oaire.citation.titleScientific Reportspt_PT
oaire.citation.volume7pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1717930b-d392-411c-8289-3ff91266a2be
relation.isProjectOfPublication6995404a-b07b-4c03-a272-7af723a52283
relation.isProjectOfPublication.latestForDiscovery1717930b-d392-411c-8289-3ff91266a2be

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