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Phylogenetic Structure and Comparative Genomics of Multi-National Invasive Haemophilus influenzae Serotype a Isolates

2022-03-24Journal article Open access
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dc.contributor.authorTopaz, Nadav
dc.contributor.authorTsang, Raymond
dc.contributor.authorDeghmane, Ala-Eddine
dc.contributor.authorClaus, Heike
dc.contributor.authorLâm, Thiên-Trí
dc.contributor.authorLitt, David
dc.contributor.authorBajanca-Lavado, Maria Paula
dc.contributor.authorPérez-Vázquez, María
dc.contributor.authorVestrheim, Didrik
dc.contributor.authorGiufrè, Maria
dc.contributor.authorVan Der Ende, Arie
dc.contributor.authorGaillot, Olivier
dc.contributor.authorKuch, Alicja
dc.contributor.authorMcElligott, Martha
dc.contributor.authorTaha, Muhamed-Kheir
dc.contributor.authorWang, Xin
dc.date.accessioned2022-12-02T15:41:45Z
dc.date.available2022-12-02T15:41:45Z
dc.date.issued2022-03-24
dc.description.abstractRecent reports have indicated a rise of invasive disease caused by Haemophilus influenzae serotype a (Hia) in North America and some European countries. The whole-genome sequences for a total of 410 invasive Hia isolates were obtained from 12 countries spanning the years of 1998 to 2019 and underwent phylogenetic and comparative genomic analysis in order to characterize the major strains causing disease and the genetic variation present among factors contributing to virulence and antimicrobial resistance. Among 410 isolate sequences received, 408 passed our quality control and underwent genomic analysis. Phylogenetic analysis revealed that the Hia isolates formed four genetically distinct clades: clade 1 (n = 336), clade 2 (n = 13), clade 3 (n = 3) and clade 4 (n = 56). A low diversity subclade 1.1 was found in clade 1 and contained almost exclusively North American isolates. The predominant sequence types in the Hia collection were ST-56 (n = 125), ST-23 (n = 98) and ST-576 (n = 51), which belonged to clade 1, and ST-62 (n = 54), which belonged to clade 4. Clades 1 and 4 contained predominantly North American isolates, and clades 2 and 3 predominantly contained European isolates. Evidence of the presence of capsule duplication was detected in clade 1 and 2 isolates. Seven of the virulence genes involved in endotoxin biosynthesis were absent from all Hia isolates. In general, the presence of known factors contributing to β-lactam antibiotic resistance was low among Hia isolates. Further tests for virulence and antibiotic susceptibility would be required to determine the impact of these variations among the isolates.pt_PT
dc.description.sponsorshipThis work was funded by Centers for Disease Control and Prevention.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationFront Microbiol. 2022 Mar 24;13:856884. doi: 10.3389/fmicb.2022.856884. eCollection 2022pt_PT
dc.identifier.doi10.3389/fmicb.2022.856884pt_PT
dc.identifier.issn1664-302X
dc.identifier.urihttp://hdl.handle.net/10400.18/8374
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherFrontiers Mediapt_PT
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fmicb.2022.856884/fullpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectHaemophilus influenzaept_PT
dc.subjectGenomicspt_PT
dc.subjectInvasive Isolatespt_PT
dc.subjectPhylogenetic Analysispt_PT
dc.subjectSerotype apt_PT
dc.subjectInfecções Respiratórias
dc.titlePhylogenetic Structure and Comparative Genomics of Multi-National Invasive Haemophilus influenzae Serotype a Isolatespt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.startPage856884pt_PT
oaire.citation.titleFrontiers in Microbiologypt_PT
oaire.citation.volume13pt_PT
rcaap.embargofctAcesso de acordo com política editorial da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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