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Brand New World of Translation

2017-05-08Conference object Restricted access
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dc.contributor.authorNeves, Ana Rita
dc.contributor.authorLacerda, Rafaela
dc.contributor.authorRomão, Luísa
dc.contributor.authorCandeias, Marco
dc.date.accessioned2018-03-02T17:22:27Z
dc.date.embargo2025-12-31
dc.date.issued2017-05-08
dc.description.abstractIn recent years, non-canonical translation initiation mechanisms have been recognized as key factors in the development of different diseases such as cancer, as they present a survival answer during stress conditions by ensuring the expression of vital proteins. Internal Ribosome Entry Sites (IRESes) were first discovered on viruses, and later in eukaryotes, as mRNA secondary structures capable of recruiting the ribosome to the vicinities of an initiation codon. One of the most studied cancer-related genes, the p53 tumor suppressor gene, was found to possess on its mRNA an IRES capable of regulating the expression of the full length isoform, p53FL, and one of its isoforms, Δ40p53 differently by the interaction with MDM2 protein, an IRES trans-acting factor (ITAF) of p53. Our aim is to study a shorter p53 protein isoform that lacks tumor suppressor behaviour acting instead as a cancer promoter (Candeias et al., 2016). One of our goals is to characterize the IRES associated with its expression. For that we will try to perform a sequencing reaction variant where the fragments to be sequenced result from uncomplete reverse transcription due to nucleotide-specific modification. Additionally, we will try to unveil new ITAFs by pulling-down the IRES and, consequently, associated factors, using two different methods: IRES biotinylation and MS2 tagging. Furthermore, we intend to find new IRESes by pulling-down MDM2 and possible bounded mRNAs followed by RNA-sequencing in order to identify them.pt_PT
dc.description.sponsorshipThis work is financed by Fundação para a Ciência e a Tecnologia (PTDC/BIMONC/4890/2014 to MMC and UID/MULTI/04046/2013 to BioISI from FCT/MCTES/PIDDAC.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/5149
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectInternal Ribosome Entry Sitespt_PT
dc.subjectExpressão Génica
dc.subjectGenómica Funcional e Estrutural
dc.titleBrand New World of Translationpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FMulti%2F04046%2F2013/PT
oaire.citation.conferencePlaceLisboa, Portugalpt_PT
oaire.citation.title2º Dia do Jovem Investigador do Instituto Nacional de Saúde Doutor Ricardo Jorge, 8 maio 2017pt_PT
oaire.fundingStream5876
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.embargofctOs resultados ainda não foram publicados.pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isProjectOfPublicationdc84f768-e6f2-4eea-b294-6c8ebbd1a156
relation.isProjectOfPublication.latestForDiscoverydc84f768-e6f2-4eea-b294-6c8ebbd1a156

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